Hull R A, Nowicki B, Kaul A, Runyan R, Svanborg C, Hull S I
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, TX 77030.
Microb Pathog. 1994 Aug;17(2):79-86. doi: 10.1006/mpat.1994.1054.
Escherichia coli FN506 containing pap genes that encode two different P fimbriae adherence specificity types were tested for virulence in a murine urinary colonization model. Strains containing adherence genes on either high copy or low copy plasmids were compared. Bacteria that harbored the adherence genes on high copy plasmids colonized mouse kidneys less well than bacteria with the same adherence genes in low copy even though the high copy strains exhibited greater hemagglutination capacity. Bacteria with either type of P fimbriae were able to colonize but pap-2+ bacteria showed increased colonizing capacity when strains containing pap-1 or pap-2 genes on low copy plasmids were compared. Bacteria containing plasmids with both adherence specificities had a similar colonizing capacity as bacteria with either type separately.
对含有编码两种不同P菌毛黏附特异性类型的pap基因的大肠杆菌FN506，在小鼠尿路定植模型中进行了毒力测试。比较了在高拷贝或低拷贝质粒上含有黏附基因的菌株。携带高拷贝质粒上黏附基因的细菌在小鼠肾脏中的定植情况不如携带相同黏附基因的低拷贝细菌，尽管高拷贝菌株表现出更强的血凝能力。当比较低拷贝质粒上含有pap-1或pap-2基因的菌株时，两种类型P菌毛的细菌都能够定植，但pap-2+细菌表现出增强的定植能力。含有两种黏附特异性质粒的细菌与单独含有任一种类型质粒的细菌具有相似的定植能力。
