Stewart C C, Stewart S J
Laboratory of Flow Cytometry, Roswell Park Cancer Institute, Buffalo, New York 14263.
Methods Cell Biol. 1994;41:61-79. doi: 10.1016/s0091-679x(08)61709-4.
The flow cytometry described can be performed using a single laser. Each laboratory has to establish its own experience base and standard operating procedures. The intent of this discussion has been to illustrate the procedures that will lead to good flow cytometry data acquisition and analysis and to illustrate problematic areas. The most important rule of all is to recognize when there is a problem. It is hoped the information provided herein will be of help in the recognition process.
所述的流式细胞术可使用单激光进行。每个实验室都必须建立自己的经验基础和标准操作程序。本次讨论的目的是说明能获得良好流式细胞术数据采集与分析结果的程序，并阐明存在问题的领域。最重要的规则是识别何时出现问题。希望本文提供的信息将有助于识别过程。
