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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Barker G R, Hardman N, Twose T M

Biochem J. 1976 Jul 1;157(1):221-7. doi: 10.1042/bj1570221.

It was shown that a system previously described for labelling R-factor DNA during transfer to an irradiated recipient strain of Escherichia coli did not allow high selectivity in the incorporation of thymine into R-factor DNA. 2. Lack of selectivity was shown to be due to cross-feeding from recipient to donor strain. 3. An improved system using a nalidixic acid-resistant recipient strain is described in which incorporation of thymine into the DNA of donor cells is minimized by addition of nalidixic acid after completion of transfer of the plasmid during conjugation.

结果表明，先前描述的用于在将R因子DNA转移至经辐照的大肠杆菌受体菌株过程中进行标记的系统，在将胸腺嘧啶掺入R因子DNA时无法实现高选择性。2. 缺乏选择性被证明是由于从受体菌株向供体菌株的交叉喂养所致。3. 本文描述了一种改进的系统，该系统使用耐萘啶酸的受体菌株，其中在接合过程中质粒转移完成后添加萘啶酸，可将胸腺嘧啶掺入供体细胞DNA的情况降至最低。

Barker G R, Hardman N, Twose T M

Biochem J. 1976 Jul 1;157(1):221-7. doi: 10.1042/bj1570221.

It was shown that a system previously described for labelling R-factor DNA during transfer to an irradiated recipient strain of Escherichia coli did not allow high selectivity in the incorporation of thymine into R-factor DNA. 2. Lack of selectivity was shown to be due to cross-feeding from recipient to donor strain. 3. An improved system using a nalidixic acid-resistant recipient strain is described in which incorporation of thymine into the DNA of donor cells is minimized by addition of nalidixic acid after completion of transfer of the plasmid during conjugation.

结果表明，先前描述的用于在将R因子DNA转移至经辐照的大肠杆菌受体菌株过程中进行标记的系统，在将胸腺嘧啶掺入R因子DNA时无法实现高选择性。2. 缺乏选择性被证明是由于从受体菌株向供体菌株的交叉喂养所致。3. 本文描述了一种改进的系统，该系统使用耐萘啶酸的受体菌株，其中在接合过程中质粒转移完成后添加萘啶酸，可将胸腺嘧啶掺入供体细胞DNA的情况降至最低。