大肠杆菌中多药耐药因子脱氧核糖核酸的复制
Replication of the deoxyribonucleic acid of multiple-drug-resistance factor in Escherichia coli.
作者信息
Barker G R, Hardman N, Twose T M
出版信息
Biochem J. 1976 Jul 1;157(1):221-7. doi: 10.1042/bj1570221.
Abstract
- It was shown that a system previously described for labelling R-factor DNA during transfer to an irradiated recipient strain of Escherichia coli did not allow high selectivity in the incorporation of thymine into R-factor DNA. 2. Lack of selectivity was shown to be due to cross-feeding from recipient to donor strain. 3. An improved system using a nalidixic acid-resistant recipient strain is described in which incorporation of thymine into the DNA of donor cells is minimized by addition of nalidixic acid after completion of transfer of the plasmid during conjugation.
摘要
- 结果表明，先前描述的用于在将R因子DNA转移至经辐照的大肠杆菌受体菌株过程中进行标记的系统，在将胸腺嘧啶掺入R因子DNA时无法实现高选择性。2. 缺乏选择性被证明是由于从受体菌株向供体菌株的交叉喂养所致。3. 本文描述了一种改进的系统，该系统使用耐萘啶酸的受体菌株，其中在接合过程中质粒转移完成后添加萘啶酸，可将胸腺嘧啶掺入供体细胞DNA的情况降至最低。
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本文引用的文献
1
Some effects of nalidixic acid on conjugation in Escherichia coli K-12.萘啶酸对大肠杆菌K-12接合作用的某些影响。
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A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.一种用于检测和分离闭环双链DNA的染料浮力密度法：HeLa细胞中的闭环DNA
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Specific labeling and physical characterization of R-factor deoxyribonucleic acid in Escherichia coli.大肠杆菌中R因子脱氧核糖核酸的特异性标记及物理特性研究
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Nalidixic acid inhibition of DNA transfer in Escherichia coli K12.萘啶酸对大肠杆菌K12中DNA转移的抑制作用。
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Studies on Escherichia coli sex factors. I. Specific labeling of F'Lac DNA.大肠杆菌性因子的研究。I. F'Lac DNA的特异性标记
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Effect of nalidixic acid on conjugational transfer and expression of episomal lac genes in Escherichia coli K12.萘啶酸对大肠杆菌K12中附加体lac基因的接合转移及表达的影响。
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Selective inhibition of semiconservative DNA synthesis by nalidixic acid in permeabilized bacteria.萘啶酸对通透化细菌中半保留DNA合成的选择性抑制作用。
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