Fenwick R G, Curtiss R
J Bacteriol. 1973 Dec;116(3):1236-46. doi: 10.1128/jb.116.3.1236-1246.1973.
During the conjugal transfer of the R64-11 plasmid at 42 C from donor cells thermosensitive for vegetative deoxyribonucleic acid (DNA) synthesis to recipient minicells, the plasmids are conjugally replicated in the donor cells. This conjugal replication is inhibited by nalidixic acid, and the degree of inhibition is comparable to the reduction in the amount of plasmid DNA transferred to the recipient minicells in the presence of the drug. In addition, the size of DNA transferred to the minicells and the fraction of conjugally replicated DNA in the donor cells that can be isolated as closed-circular plasmid DNA under alkaline conditions are both reduced by nalidixic acid. When the drug is added to a mating that is underway, the rate of conjugal replication is immediately reduced. This change is accompanied by a reduction in the amount of conjugally replicated DNA in the donor cells that can be isolated as closed-circular plasmid DNA. Furthermore, conjugally replicated plasmid DNA that is not associated with the donor cell membrane becomes membrane bound after the addition of nalidixic acid.
在42℃下，R64 - 11质粒从对营养型脱氧核糖核酸（DNA）合成敏感的供体细胞向受体微小细胞进行接合转移时，质粒在供体细胞中进行接合复制。这种接合复制受到萘啶酸的抑制，抑制程度与在药物存在下转移到受体微小细胞中的质粒DNA量的减少程度相当。此外，转移到微小细胞中的DNA大小以及在碱性条件下可作为闭环质粒DNA分离的供体细胞中接合复制DNA的比例，都因萘啶酸而降低。当药物添加到正在进行的交配中时，接合复制速率立即降低。这种变化伴随着供体细胞中可作为闭环质粒DNA分离的接合复制DNA量的减少。此外，添加萘啶酸后，未与供体细胞膜结合的接合复制质粒DNA会与膜结合。
