Collavo D, Biasi G, Colombatti A, Chieco-Bianchi L
Br J Cancer. 1975 Sep;32(3):331-8. doi: 10.1038/bjc.1975.231.
To investigate whether AKR spontaneous leukaemogenesis is associated with a reduction in functional activity of T lymphocytes, the PHA response of AKR blood cells at different ages up to and including the preleukaemic period was studied. No significant differences were observed among young, adult and preleukaemic donors. In addition, the in vitro and in vivo AKR lymphocyte functions were compared with those of CBA lymphocytes by means of their response to stimulation with T and B lymphocyte selective mitogens (PHA, Con A and LSP respectively), and their response to immunization with thymus dependent (SRBC) or independent (LPS) antigens. We observed in vitro that while the B lymphocytes responded normally to mitogen, an intrinsic hyporeactivity to mitogens characterizes the T lymphocytes. Moreover, AKR mice exhibited a reduced in vivo response to both thymus dependent and independent antigens.
为了研究AKR自发性白血病发生是否与T淋巴细胞功能活性降低有关，我们研究了直至白血病前期（包括该时期）不同年龄的AKR血细胞对PHA的反应。在年轻、成年和白血病前期供体之间未观察到显著差异。此外，通过AKR淋巴细胞对T和B淋巴细胞选择性有丝分裂原（分别为PHA、Con A和LSP）刺激的反应以及它们对胸腺依赖性（SRBC）或非胸腺依赖性（LPS）抗原免疫的反应，将AKR淋巴细胞的体内外功能与CBA淋巴细胞的功能进行了比较。我们在体外观察到，虽然B淋巴细胞对有丝分裂原反应正常，但T淋巴细胞的特征是对有丝分裂原存在内在低反应性。此外，AKR小鼠对胸腺依赖性和非胸腺依赖性抗原的体内反应均降低。
