Nakamura E, Mizuta H, Otsuka Y, Mizumoto Y, Takagi K
Department of Orthopedics, Kumamoto University School of Medicine, Japan.
Acta Orthop Scand. 1995 Feb;66(1):33-7. doi: 10.3109/17453679508994635.
We investigated the effects of tibial lengthening by callotasis on glycosaminoglycan (GAG) metabolism of the knee articular cartilage in 30 rabbits. The distraction rate was 1 mm per day. On the right side, the daily distraction was in 2 steps, while on the left it was in 120 steps. The animals were divided into 3 subgroups based on length gain; 10, 20, and 30 percent, respectively. The knee joint fluid and medial tibial cartilage were examined by quantitative analysis of the GAG content and/or synthesis. The immunoreactivity for chondroitin sulfate in the cartilage was also examined by immunohistochemistry. For all length gains, the GAG concentration in the synovial fluid was higher on both sides than in controls, with no difference between sides. The GAG content and synthesis in the cartilage on the 2-step side decreased gradually with increasing length. On the 120-step side, the content did not differ from control values in any length gain, and the level of synthesis at 20 and 30 percent lengthening was higher than the control level. Our findings indicate that the alterations in GAG metabolism are attributable to increased mechanical stress on the articular cartilage, suggesting a moderate increase on the 120-step side compared to an excessive one on the 2-step side.
我们研究了骨痂延长术对30只兔子膝关节软骨糖胺聚糖（GAG）代谢的影响。牵张速率为每天1毫米。右侧每天分2步进行牵张，而左侧分120步进行。根据长度增加量将动物分为3个亚组，分别为增加10%、20%和30%。通过对GAG含量和/或合成进行定量分析来检测膝关节液和胫骨内侧软骨。还通过免疫组织化学检测软骨中硫酸软骨素的免疫反应性。对于所有长度增加量，两侧滑液中的GAG浓度均高于对照组，且两侧之间无差异。2步侧软骨中的GAG含量和合成随着长度增加而逐渐降低。在120步侧，在任何长度增加量下其含量与对照值均无差异，且在延长20%和30%时的合成水平高于对照水平。我们的研究结果表明，GAG代谢的改变归因于关节软骨上机械应力的增加，这表明与2步侧的过度应力相比，120步侧的应力适度增加。
