Fang Y, Ye W X, Nei H Y, Wang Y L
Huadong Research Institute for Medical Biotechnics, Nanjing, People's Republic of China.
Acta Trop. 1994 Oct;58(1):79-83. doi: 10.1016/0001-706x(94)90123-6.
By exposure to gradually increasing concentrations of suramin in modified Baltz's cell free culture system, suramin-resistant clones of Trypanosoma evansi, JGcl-160, JX-lcl-160 and ZJcl-140, were derived from suramin-sensitive clones JGcl, JX-lcl and ZJcl, respectively, over a period of 550 days. In vitro tests showed that the IC50 values of JGcl-160, JX-lcl-160 and ZJcl-140 were 358.5, 412.3 and 246.4 micrograms/ml respectively, which were 1792.5, 1874.1 and 1760.0 times higher than those of their parent clones. The values for the curative dose CD100 for intact mice infected with JGcl-160, JX-lcl-160 and ZJcl-140 were 80, 120 and 30 mg/kg respectively, which were 5.3, 8.0 and 3.0 times higher than those of each parent clone. The CD100 values for immunosuppressed mice infected with these clones were 250, 300 and 100 mg/kg, respectively, which were 3.1, 2.5 and 3.3 times higher those for intact mice infected with the same clones.
在改良的巴尔茨无细胞培养系统中，通过逐渐增加苏拉明的浓度进行处理，在550天的时间里，分别从伊氏锥虫的苏拉明敏感克隆JGcl、JX - lcl和ZJcl中获得了抗苏拉明的克隆JGcl - 160、JX - lcl - 160和ZJcl - 140。体外试验表明，JGcl - 160、JX - lcl - 160和ZJcl - 140的半数抑制浓度（IC50）值分别为358.5、412.3和246.4微克/毫升，分别是其亲代克隆的1792.5、1874.1和1760.0倍。感染JGcl - 160、JX - lcl - 160和ZJcl - 140的完整小鼠的治愈剂量（CD100）值分别为80、120和30毫克/千克，分别是每个亲代克隆的5.3、8.0和3.0倍。感染这些克隆的免疫抑制小鼠的CD100值分别为250、300和100毫克/千克，分别是感染相同克隆的完整小鼠的3.1、2.5和3.3倍。
