Chen S L, Gutmains F
Biotechnol Bioeng. 1976 Oct;18(10):1455-62. doi: 10.1002/bit.260181012.
Saccharomyces cerevisiae was grown under aerobic and substrate-limiting conditions for efficient biomass production. Under these conditions, where the sugar substrate was fed incrementally, the growth pattern of the yeast cells was found to be uniform, as indicated by a constant respiratory quotient during the entire growing period. The effect of carbon dioxide was investigated by replacing portions of the nitrogen in the air stream with carbon dioxide, while maintaining the oxygen content at the normal 20% level, so that identical oxygen transfer rate and atmospheric pressure were maintained for all experiments with different partial pressures of carbon dioxide. Inhibition of yeast growth was negligible below 20% CO2 in the aeration mixture. Slight inhibition was noted at the 40% CO2 level and significant inhibition was noted above the 50% CO2 level, corresponding to 1.6 X 10(-2)M of dissolved CO2 in the fermentor broth. High carbon dioxide content in the gas phase also inhibited the fermentation activity of baker's yeast.
酿酒酵母在有氧和底物限制条件下培养以实现高效的生物质生产。在这些条件下，糖底物逐步进料，发现酵母细胞的生长模式是均匀的，这在整个生长期间通过恒定的呼吸商来表明。通过用二氧化碳替代气流中的部分氮气来研究二氧化碳的影响，同时将氧气含量保持在正常的20%水平，以便在不同二氧化碳分压的所有实验中维持相同的氧传递速率和大气压。在曝气混合物中二氧化碳含量低于20%时，对酵母生长的抑制作用可忽略不计。在40%二氧化碳水平时观察到轻微抑制，在50%二氧化碳水平以上观察到显著抑制，这对应于发酵罐肉汤中1.6×10(-2)M的溶解二氧化碳。气相中高含量的二氧化碳也抑制了面包酵母的发酵活性。
