Seip R L, Angelopoulos T J, Semenkovich C F
Department of Medicine, Washington University School of Medicine, St. Louis, Missouri 63110.
Am J Physiol. 1995 Feb;268(2 Pt 1):E229-36. doi: 10.1152/ajpendo.1995.268.2.E229.
Lipoprotein lipase (LPL) is regulated by exercise in humans, but the effects of exercise on LPL expression in different tissues and the molecular mechanisms involved are unclear. We assessed the effects of 5-13 consecutive days of supervised exercise on tissue LPL expression as well as fasting plasma lipids and lipoproteins in 32 sedentary, weight-stable adult men. In skeletal muscle, exercise training increased the mean LPL mRNA level by 117% (P = 0.037), LPL protein mass by 53% (P = 0.038), and total LPL enzyme activity by 35% (P = 0.025). In adipose tissue, mean LPL mRNA, protein mass, and activity did not change. Exercise decreased triglycerides [from 172 +/- 4.3 to 127 +/- 3.2 (SE) mg/dl, P = 0.002], total cholesterol (from 188 +/- 1.2 to 181 +/- 1.0 mg/dl, P = 0.011), and very low-density lipoprotein-cholesterol (from 30.1 +/- 0.9 to 22.0 +/- 0.8, P = 0.004) and increased high-density lipoprotein cholesterol (HDL-C; from 43.4 +/- 0.35 to 45.0 +/- 0.37, P = 0.030) and HDL2-C (from 6.6 +/- 0.21 to 7.7 +/- 0.19, P = 0.021). Changes in muscle but not adipose tissue heparin-releasable LPL activity were inversely correlated (r = -0.435, P < 0.034) with changes in triglycerides. These data suggest the existence of an exercise stimulus intrinsic to skeletal muscle, which raises LPL activity in part by pretranslational mechanisms, a process that contributes to the improvement in circulating lipids seen with physical activity.
脂蛋白脂肪酶（LPL）在人体中受运动调节，但其对不同组织中LPL表达的影响以及相关分子机制尚不清楚。我们评估了32名久坐不动、体重稳定的成年男性连续5 - 13天在监督下进行运动对组织LPL表达以及空腹血脂和脂蛋白的影响。在骨骼肌中，运动训练使LPL mRNA平均水平提高了117%（P = 0.037），LPL蛋白量增加了53%（P = 0.038），总LPL酶活性提高了35%（P = 0.025）。在脂肪组织中，LPL mRNA、蛋白量和活性的平均值没有变化。运动使甘油三酯水平降低[从172±4.3降至127±3.2（SE）mg/dl，P = 0.002]，总胆固醇水平降低（从188±1.2降至181±1.0 mg/dl，P = 0.011），极低密度脂蛋白胆固醇水平降低（从30.1±0.9降至22.0±0.8，P = 0.004），并使高密度脂蛋白胆固醇（HDL-C）水平升高（从43.4±0.35升至45.0±0.37，P = 0.030）以及HDL2-C水平升高（从6.6±0.21升至7.7±0.19，P = 0.021）。肌肉而非脂肪组织中肝素可释放的LPL活性变化与甘油三酯变化呈负相关（r = -0.435，P < 0.034）。这些数据表明骨骼肌存在一种运动刺激，它部分通过翻译前机制提高LPL活性，这一过程有助于改善体育活动时的循环脂质水平。
