使用具有延时积分功能的电荷耦合器件在毛细管区带电泳中进行荧光检测。

Fluorescence detection in capillary zone electrophoresis using a charge-coupled device with time-delayed integration.

作者信息

Sweedler J V, Shear J B, Fishman H A, Zare R N, Scheller R H

机构信息

Department of Chemistry, Stanford University, California 94305.

出版信息

Anal Chem. 1991 Mar 1;63(5):496-502. doi: 10.1021/ac00005a022.

DOI:10.1021/ac00005a022

PMID:1905883

Abstract

A fluorescence detection system for capillary zone electrophoresis is described in which a charged-coupled device (CCD) views a 2-cm section of an axially illuminated capillary column. The CCD is operated in two readout modes: a snapshot mode that acquires a series of images in wavelength and capillary position, and a time-delayed integration mode that allows long exposure times of the moving analyte zones. By use of the latter mode, the ability to differentiate a species based on both its fluorescence emission and migration rate is demonstrated for fluorescein and sulforhodamine 101. The detection limit for fluorescein isothiocyanate (FITC) is 1.2 X 10(-20) mol; detection limits for FITC-amino acids are in the (2-8) X 10(-20) mol range.

摘要

描述了一种用于毛细管区带电泳的荧光检测系统，其中电荷耦合器件（CCD）观察轴向照明的毛细管柱的2厘米部分。CCD以两种读出模式运行：一种快照模式，可在波长和毛细管位置获取一系列图像；另一种延时积分模式，可对移动的分析物区进行长时间曝光。通过使用后一种模式，展示了基于荧光发射和迁移速率区分荧光素和磺基罗丹明101这两种物质的能力。异硫氰酸荧光素（FITC）的检测限为1.2×10^(-20)摩尔；FITC-氨基酸的检测限在(2 - 8)×10^(-20)摩尔范围内。