Carrier M, Thai P, Pelletier L C
Montreal Heart Institute, Quebec, Canada.
Transpl Int. 1994;7(6):388-91. doi: 10.1007/BF00346031.
To evaluate the effect of cyclosporin (CyA) on the mesenteric arterial bed, studies were performed on the isolated mesenteric artery perfused at a constant flow in 20 dogs. Changes in mesenteric perfusion pressure reflected variations in vascular resistance. Pure powder CyA was dissolved in autologous blood and injected at doses of 5, 10, 20 and 40 mg. Infusions of 5 and 10 mg CyA caused nonsignificant mean increases of 3 +/- 2 mm Hg [95% confidence interval (CI) -2 to +7; P > 0.05] and 3 +/- 3 mm Hg (95% CI -3 to +9; P > 0.05) in mesenteric perfusion pressure, with CyA blood levels in the mesenteric vein averaging 466 +/- 153 and 692 +/- 130 nmol/l, respectively, at the end of the injections. Infusions of 20 and 40 mg CyA caused significant increases in mesenteric perfusion pressure averaging 11 +/- 3 mm Hg (95% CI 3-18; P < 0.05) and 26 +/- 4 mm Hg (95% CI 16-34; P < 0.05), respectively. CyA blood levels at the end of infusion averaged 806 +/- 85 and 1118 +/- 89 nmol/l, respectively, in the mesenteric vein. Blockade of alpha-adrenergic receptors with phentolamine abolished the CyA vasoconstriction of the mesenteric artery, with the increase in perfusion pressure averaging 16 +/- 4 mm Hg before and 3 +/- 3 mm Hg after phentolamine (P < 0.05). Thus, in the dog, CyA causes an acute vasoconstriction of the mesenteric artery through stimulation of alpha-adrenergic receptors.
为评估环孢素（CyA）对肠系膜动脉床的作用，对20只犬的离体肠系膜动脉进行了恒流灌注研究。肠系膜灌注压的变化反映了血管阻力的变化。将纯粉状CyA溶解于自体血中，以5、10、20和40mg的剂量注射。输注5mg和10mg CyA导致肠系膜灌注压平均分别非显著升高3±2mmHg[95%置信区间（CI）-2至+7；P>0.05]和3±3mmHg（95%CI-3至+9；P>0.05），注射结束时肠系膜静脉中CyA血药浓度分别平均为466±153和692±130nmol/L。输注20mg和40mg CyA导致肠系膜灌注压显著升高，平均分别为11±3mmHg（95%CI 3-18；P<0.05）和26±4mmHg（95%CI 16-34；P<0.05）。输注结束时肠系膜静脉中CyA血药浓度分别平均为806±85和1118±89nmol/L。用酚妥拉明阻断α-肾上腺素能受体可消除CyA对肠系膜动脉的血管收缩作用，酚妥拉明给药前灌注压平均升高16±4mmHg，给药后为3±3mmHg（P<0.05）。因此，在犬体内，CyA通过刺激α-肾上腺素能受体引起肠系膜动脉急性血管收缩。
