Dierynck I, Bernard A, Roels H, De Ley M
Laboratory of Biochemistry, Catholic University of Leuven, Belgium.
Am J Respir Cell Mol Biol. 1995 Feb;12(2):205-10. doi: 10.1165/ajrcmb.12.2.7865218.
The Clara cell 16 kD protein (CC16), the predominant product of the Clara cells lining the bronchiolar epithelium, is thought to protect the respiratory and urogenital tract from unwanted inflammatory reactions through its immunosuppressive action. In this report, we show evidence that CC16 establishes an anti-inflammatory activity by interfering with the interferon-gamma (IFN-gamma)-mediated actions of the cytokine network. The HuIFN-gamma production of stimulated single-donor peripheral blood mononuclear cells is inhibited by the presence of doses of CC16 in the range of 10(-12) M, with a maximal inhibition (up to 95%) when interleukin-2 is used as a stimulating agent. CC16 also diminishes the biologic activity of IFN-gamma: both the antiviral activity and the stimulation of phagocytosis by IFN-gamma, measured by means of chemiluminescence, are reduced in the presence of CC16. These observations indicate that CC16 acts as an anticytokine and could give new insight in the potential role of the Clara cells.
克拉拉细胞16kD蛋白（CC16）是支气管上皮内衬克拉拉细胞的主要产物，被认为可通过其免疫抑制作用保护呼吸道和泌尿生殖道免受不必要的炎症反应。在本报告中，我们提供证据表明CC16通过干扰细胞因子网络的干扰素-γ（IFN-γ）介导的作用来建立抗炎活性。剂量在10^(-12)M范围内的CC16可抑制刺激后的单供体外周血单个核细胞产生人IFN-γ，当使用白细胞介素-2作为刺激剂时，抑制作用最大（高达95%）。CC16还可降低IFN-γ的生物活性：通过化学发光法测定，CC16存在时，IFN-γ的抗病毒活性和对吞噬作用的刺激均降低。这些观察结果表明CC16作为一种抗细胞因子发挥作用，并可能为克拉拉细胞的潜在作用提供新的见解。
