O'Keeffe M A, Metcalfe S A, Glew M D, Bowden T, McInnes S, Kimpton W G, Cahill R N, Hein W R, Walker I D
Department of Veterinary Science, University of Melbourne, Parkville, Victoria, Australia.
Int Immunol. 1994 Nov;6(11):1687-97. doi: 10.1093/intimm/6.11.1687.
Sheep gamma delta T cells have been shown serologically to express T19, a membrane protein of 180-200 kDa which is a member of the scavenger receptor superfamily. Previous work from this laboratory resulted in the detection of a multigene family of T19-like genes in the sheep genome. In this study nucleotide sequences from several T19 genes were determined and are reported along with the corresponding segments of a number of expressed mRNA molecules. A segment of a single sheep T19-like gene was sequenced and these data, along with the corresponding sequences from cloned T19-like cDNA molecules from sheep and cow, were used to design an oligonucleotide primer system suitable for amplification of corresponding segments of many T19 genes and their cDNAs. Between 30 and 40% of cloned T19 genes were amenable to amplification using the selected primers, and sequence analysis of cloned PCR products confirmed that different T19 genes encode unique amino acid sequences. The expression of multiple T19 genes was established using cDNA molecules obtained from a single sample of sheep lymphocyte mRNA. The possible role of the T19 family of genes is discussed.
血清学研究表明，绵羊γδ T细胞表达T19，这是一种分子量为180 - 200 kDa的膜蛋白，属于清道夫受体超家族成员。该实验室之前的研究在绵羊基因组中检测到了一个T19样基因的多基因家族。在本研究中，测定了多个T19基因的核苷酸序列，并与一些表达的mRNA分子的相应片段一同报告。对单个绵羊T19样基因的一个片段进行了测序，这些数据以及来自绵羊和牛的克隆T19样cDNA分子的相应序列，被用于设计一个寡核苷酸引物系统，该系统适用于扩增许多T19基因及其cDNA的相应片段。使用选定的引物，30%至40%的克隆T19基因可被扩增，对克隆的PCR产物进行序列分析证实，不同的T19基因编码独特的氨基酸序列。利用从单个绵羊淋巴细胞mRNA样本中获得的cDNA分子，确定了多个T19基因的表达情况。文中还讨论了T19基因家族可能的作用。
