人结肠癌细胞系（SW480细胞）和猫肾细胞系（CRFK细胞）中各种慢病毒长末端重复序列的比较功能分析。

Comparative functional analysis of the various lentivirus long terminal repeats in human colon carcinoma cell line (SW480 cells) and feline renal cell line (CRFK cells).

作者信息

Miyazawa T, Kawaguchi Y, Kohmoto M, Tomonaga K, Mikami T

机构信息

Department of Veterinary Microbiology, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

J Vet Med Sci. 1994 Oct;56(5):895-9. doi: 10.1292/jvms.56.895.

DOI:10.1292/jvms.56.895

PMID:7865591

Abstract

Basal promoter activities of various lentiviral long terminal repeats (LTRs) in a human colon carcinoma cell line (SW480 cells) and a feline renal cell line (CRFK cells) were examined by the chloramphenicol acetyltransferase (CAT) assay using the LTR-CAT reporter plasmids. In SW480 cells, the basal promoter activities induced by LTRs of visna virus, caprine arthritis-encephalitis virus (CAEV), and simian immunodeficiency virus (SIVAGM) were moderate, and those induced by LTRs of human immunodeficiency virus (HIV) type 1 (HIV-1) and HIV type 2 (HIV-2) were low. However, the activity induced by the LTR of feline immunodeficiency virus (FIV) was extremely low. In CRFK cells, the basal promoter activities induced by LTRs of visna virus, CAEV and SIVAGM were relatively high, and those induced by LTRs of HIV-1, HIV-2 and FIV were moderate. From these data, although the structure of the LTR of FIV is reported to be similar to that of visna virus and CAEV, the function of the LTR of FIV is rather quite different from that of the LTR of these viruses.

摘要

使用LTR-CAT报告质粒，通过氯霉素乙酰转移酶（CAT）测定法检测了人结肠癌细胞系（SW480细胞）和猫肾细胞系（CRFK细胞）中各种慢病毒长末端重复序列（LTR）的基础启动子活性。在SW480细胞中，维斯纳病毒、山羊关节炎-脑炎病毒（CAEV）和猴免疫缺陷病毒（SIVAGM）的LTR诱导的基础启动子活性中等，而1型人类免疫缺陷病毒（HIV-1）和2型人类免疫缺陷病毒（HIV-2）的LTR诱导的活性较低。然而，猫免疫缺陷病毒（FIV）的LTR诱导的活性极低。在CRFK细胞中，维斯纳病毒、CAEV和SIVAGM的LTR诱导的基础启动子活性相对较高，而HIV-1、HIV-2和FIV的LTR诱导的活性中等。从这些数据来看，尽管据报道FIV的LTR结构与维斯纳病毒和CAEV的相似，但其LTR的功能与这些病毒的LTR相当不同。