Sakuragi J, Fukasawa M, Shibata R, Sakai H, Kawamura M, Akari H, Kiyomasu T, Ishimoto A, Hayami M, Adachi A
Department of Viral Oncology, Kyoto University, Japan.
Virology. 1991 Nov;185(1):455-9. doi: 10.1016/0042-6822(91)90798-g.
The promoter activity of long terminal repeats (LTRs) of four strains of the simian immunodeficiency virus isolated from African green monkeys (SIVAGM) was compared with those of various LTRs derived from the other representative primate lentiviruses: human immunodeficiency virus type 1 (HIV-1), type 2 (HIV-2), SIV from a rhesus monkey (SIVMAC), and SIV from a mandrill (SIVMND). The expression of the LTRs was evaluated by monitoring chloramphenicol acetyltransferase production after transfection of reporter plasmid clones. In the absence of viral tat, all SIVAGM LTRs acted as much more efficient promoters than any of the other LTRs. When tat gene products were supplied in trans, LTRs of SIVAGM and SIVMND were activated inefficiently relative to high responder LTRs of HIV-2 and SIVMAC. The LTR of HIV-1 was highly activated by HIV-1 tat, but not so much by HIV-2, SIVAGM, and SIVMND tat.
比较了从非洲绿猴分离出的四株猴免疫缺陷病毒（SIVAGM）的长末端重复序列（LTR）的启动子活性与源自其他代表性灵长类慢病毒的各种LTR的启动子活性：1型人类免疫缺陷病毒（HIV-1）、2型（HIV-2）、恒河猴的SIV（SIVMAC）和山魈的SIV（SIVMND）。通过监测报告质粒克隆转染后氯霉素乙酰转移酶的产生来评估LTR的表达。在没有病毒tat的情况下，所有SIVAGM LTR作为启动子比任何其他LTR更有效。当反式提供tat基因产物时，相对于HIV-2和SIVMAC的高反应性LTR，SIVAGM和SIVMND的LTR激活效率较低。HIV-1的LTR被HIV-1 tat高度激活，但被HIV-2、SIVAGM和SIVMND tat激活的程度不高。
