Wu W, Qiu F
Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan.
Chin Med Sci J. 1994 Sep;9(3):162-6.
The present study was to investigate changes of free radicals in the whole rat liver, changes of ATP levels of hepatic cells, ultrastructural changes in hepatic tissue during ischemia and reperfusion in rats and the effects of ligustrazine and salvia compound. The results indicated that: 1) the free radical levels increased by 8.3-fold and 9.0-fold in the groups with 30 and 60 min ischemia after 40 min reperfusion, as compared with the group with ischemia only (P < 0.01, P < 0.01, respectively). 2) The levels of ATP returned to normal in the group with 30 min ischemia after reperfusion for 40 min and 3 d, remained low in the group with 90 min ischemia and fell again after a mild increase in the group with 60 min ischemia. 3) The hepatic and endothelial cell damage after ischemia became more severe after reperfusion, as revealed by electron microscopy. The present study also showed that ligustrazine and salvia compound have protective effects against reperfusion injury. They can be used to scavenge free radicals, improve hepatic microcirculation and alleviate hepatic and endothelial cell damage.
本研究旨在探讨大鼠肝脏缺血再灌注过程中全肝自由基变化、肝细胞ATP水平变化、肝组织超微结构变化以及川芎嗪和复方丹参的作用。结果表明：1）再灌注40分钟后，缺血30分钟和60分钟组的自由基水平分别比单纯缺血组升高了8.3倍和9.0倍（P均<0.01）。2）缺血30分钟组再灌注40分钟及3天后ATP水平恢复正常，缺血90分钟组ATP水平仍较低，缺血60分钟组ATP水平轻度升高后又再次下降。3）电镜显示，缺血后肝组织及内皮细胞损伤在再灌注后加重。本研究还表明，川芎嗪和复方丹参对再灌注损伤有保护作用。它们可用于清除自由基、改善肝脏微循环并减轻肝组织及内皮细胞损伤。
