Coussons-Read M E, Dykstra L A, Lysle D T
Department of Psychology, University of North Carolina at Chapel Hill 27599-3270.
Brain Behav Immun. 1994 Sep;8(3):204-17. doi: 10.1006/brbi.1994.1019.
The present studies examined the involvement of peripheral beta-adrenergic receptor activity in the establishment and expression of conditioned morphine-induced alterations of immune status. Previous work in our laboratory has shown that morphine's immunomodulatory effects can become conditioned to environmental stimuli which predict drug administration. These immune alterations include conditioned changes in natural killer cell activity, interleukin-2 production, and mitogen-induced lymphocyte proliferation. During the training phase of these experiments, Lewis rats received two conditioning sessions during which a subcutaneous injection of 15 mg/kg morphine sulfate was paired with exposure to a distinctive environment. On the test day, rats were reexposed to the conditioned stimulus prior to sacrifice. Saline or nadolol (0.002, 0.02, 0.2, or 2.0 mg/kg) was administered either prior to the training sessions or prior to the test session. Administration of nadolol prior to training did not affect the development of conditioned alterations of immune status. Conversely, nadolol administration prior to testing completely attenuated the expression of a subset of the conditioned morphine-induced changes in immune status. Taken together, these studies suggest that whereas peripheral beta-adrenergic receptor activity is not required for the establishment of conditioned morphine-induced alterations of immune status, it is involved in the expression of a subset of these conditioned immunomodulatory effects.
本研究检测了外周β-肾上腺素能受体活性在条件性吗啡诱导的免疫状态改变的建立和表达中的作用。我们实验室之前的工作表明，吗啡的免疫调节作用可被条件化为预测药物给药的环境刺激。这些免疫改变包括自然杀伤细胞活性、白细胞介素-2产生以及丝裂原诱导的淋巴细胞增殖的条件性变化。在这些实验的训练阶段，Lewis大鼠接受两次条件训练，期间皮下注射15mg/kg硫酸吗啡并同时暴露于一个独特的环境中。在测试日，大鼠在处死前再次暴露于条件刺激下。在训练前或测试前给予生理盐水或纳多洛尔（0.002、0.02、0.2或2.0mg/kg）。训练前给予纳多洛尔不影响免疫状态条件性改变的形成。相反，测试前给予纳多洛尔完全减弱了条件性吗啡诱导的免疫状态变化的一个子集的表达。综上所述，这些研究表明，虽然外周β-肾上腺素能受体活性对于条件性吗啡诱导的免疫状态改变的建立不是必需的，但它参与了这些条件性免疫调节作用的一个子集的表达。
