一种拟南芥热休克蛋白弥补了酵母中的耐热缺陷。
An Arabidopsis heat shock protein complements a thermotolerance defect in yeast.
作者信息
Schirmer E C, Lindquist S, Vierling E
机构信息
Department of Molecular Genetics and Cell Biology, University of Chicago, Illinois 60637.
出版信息
Plant Cell. 1994 Dec;6(12):1899-909. doi: 10.1105/tpc.6.12.1899.
Abstract
The heat shock protein Hsp104 of the yeast Saccharomyces cerevisiae plays a key role in promoting survival at extreme temperatures. We found that when diverse higher plant species are exposed to high temperatures they accumulate proteins that are antigenically related to Hsp104. We isolated a cDNA corresponding to one of these proteins from Arabidopsis. The protein, AtHSP101, is 43% identical to yeast Hsp104. DNA gel blot analysis indicated that AtHSP101 is encoded by a single- or low-copy number gene. AtHsp101 mRNA was undetectable in the absence of stress but accumulated to high levels during exposure to high temperatures. When AtHSP101 was expressed in yeast, it complemented the thermotolerance defect caused by a deletion of the HSP104 gene. The ability of AtHSP101 to protect yeast from severe heat stress strongly suggests that this HSP plays an important role in thermotolerance in higher plants.
摘要
酿酒酵母的热休克蛋白Hsp104在促进极端温度下的存活中起关键作用。我们发现，当多种高等植物物种暴露于高温时，它们会积累与Hsp104抗原相关的蛋白质。我们从拟南芥中分离出一个与这些蛋白质之一对应的cDNA。该蛋白质AtHSP101与酵母Hsp104有43%的同一性。DNA凝胶印迹分析表明AtHSP101由一个单拷贝或低拷贝数基因编码。在没有胁迫的情况下检测不到AtHsp101 mRNA，但在高温暴露期间积累到高水平。当AtHSP101在酵母中表达时，它弥补了由HSP104基因缺失引起的耐热性缺陷。AtHSP101保护酵母免受严重热胁迫的能力强烈表明，这种热休克蛋白在高等植物的耐热性中起重要作用。
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本文引用的文献
1
A class of soybean low molecular weight heat shock proteins : immunological study and quantitation.一类大豆小分子热休克蛋白：免疫研究与定量。
Plant Physiol. 1992 Aug;99(4):1279-84. doi: 10.1104/pp.99.4.1279.
2
Thermotolerance is developmentally dependent in germinating wheat seed.热耐受性在萌发的小麦种子中具有发育依赖性。
Plant Physiol. 1989 Feb;89(2):569-76. doi: 10.1104/pp.89.2.569.
3
Ribulose 1,5-Bisphosphate Carboxylase Synthesis during Heat Shock.热休克期间1,5-二磷酸核酮糖羧化酶的合成
Plant Physiol. 1985 May;78(1):155-62. doi: 10.1104/pp.78.1.155.
4
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J Bacteriol. 1993 Oct;175(20):6484-91. doi: 10.1128/jb.175.20.6484-6491.1993.
5
Antibodies raised against yeast HSP 104 cross-react with a heat- and abscisic acid-regulated polypeptide in rice.针对酵母HSP 104产生的抗体与水稻中一种受热和脱落酸调节的多肽发生交叉反应。
Plant Mol Biol. 1993 Sep;22(6):1177-80. doi: 10.1007/BF00028989.
6
Poly(A) tail length of a heat shock protein RNA is increased by severe heat stress, but intron splicing is unaffected.热休克蛋白RNA的聚腺苷酸尾长度在严重热应激下会增加，但内含子剪接不受影响。
Mol Gen Genet. 1993 Jun;239(3):323-33. doi: 10.1007/BF00276930.
7
The function of heat-shock proteins in stress tolerance: degradation and reactivation of damaged proteins.热休克蛋白在应激耐受中的作用：受损蛋白质的降解与再激活。
Annu Rev Genet. 1993;27:437-96. doi: 10.1146/annurev.ge.27.120193.002253.
8
A soybean 101-kD heat shock protein complements a yeast HSP104 deletion mutant in acquiring thermotolerance.一种大豆101-kD热休克蛋白在获得耐热性方面可补充酵母HSP104缺失突变体。
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9
Characterization of cDNA for a dehydration-inducible gene that encodes a CLP A, B-like protein in Arabidopsis thaliana L.拟南芥中一个编码CLP A、B样蛋白的脱水诱导基因的cDNA特性分析
Biochem Biophys Res Commun. 1993 Nov 15;196(3):1214-20. doi: 10.1006/bbrc.1993.2381.
10
Rapid isolation of high molecular weight plant DNA.高分子量植物DNA的快速分离
Nucleic Acids Res. 1980 Oct 10;8(19):4321-5. doi: 10.1093/nar/8.19.4321.
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