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来自面包酵母的非起始甲硫氨酸转移核糖核酸的一级结构。II. 用核糖核酸酶T1进行部分消化及完整序列的推导

The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. II. Partial digestion with ribonuclease T1 and derivation of the complete sequence.

作者信息

Koiwai O, Miyazaki M

出版信息

J Biochem. 1976 Nov;80(5):951-9. doi: 10.1093/oxfordjournals.jbchem.a131382.

DOI:10.1093/oxfordjournals.jbchem.a131382
PMID:826525
Abstract

Large oligonucleotides obtained from partial RNase T1 digestion of tRNAMetm from bakers' yeast (S. cerevisiae, strain Y 185) were isolated by chromatographic procedures and sequenced. The complete sequence of the tRNAMetm was established from the results of this partial digestion, together with those of the complete RNase T1 and A [EC 3.1.4.8 and 22] digestions of tRNAMetm reported in the preceding paper. The structure of this tRNAMetm was arranged into a clover leaf comparable with those of other tRNAMet species including bakers' yeast initiator tRNA.

摘要

从面包酵母(酿酒酵母,Y185菌株)的tRNAMetm经部分核糖核酸酶T1消化获得的大的寡核苷酸,通过色谱法进行分离并测序。根据这次部分消化的结果,以及在前一篇论文中报道的tRNAMetm经核糖核酸酶T1和A[EC 3.1.4.8和22]完全消化的结果,确定了tRNAMetm的完整序列。该tRNAMetm的结构排列成一个三叶草叶状,与包括面包酵母起始tRNA在内的其他tRNAMet种类的结构相似。

相似文献

1
The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. II. Partial digestion with ribonuclease T1 and derivation of the complete sequence.来自面包酵母的非起始甲硫氨酸转移核糖核酸的一级结构。II. 用核糖核酸酶T1进行部分消化及完整序列的推导
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2
The primary structure of non-initiator methionine transfer ribonucleic acid from Bakers' yeast. I. Purification and complete digestion with ribonuclease T1 and pancreatic ribonuclease A.来自面包酵母的非起始甲硫氨酸转移核糖核酸的一级结构。I. 用核糖核酸酶T1和胰核糖核酸酶A进行纯化及完全消化。
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The nucleotide sequence of cysteine transfer ribonucleic acid from baker's yeast. Products of complete digestion with pancreatic ribonuclease and ribonuclease T1.面包酵母半胱氨酸转移核糖核酸的核苷酸序列。用胰核糖核酸酶和核糖核酸酶T1完全消化的产物。
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引用本文的文献

1
The nucleotide sequence of spinach chloroplast methionine elongator tRNA.菠菜叶绿体甲硫氨酸延伸tRNA的核苷酸序列。
Nucleic Acids Res. 1981 Jan 10;9(1):183-8. doi: 10.1093/nar/9.1.183.