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酵母非起始甲硫氨酸转运RNA基因的结构

Structure of a yeast non-initiating methionine-tRNA gene.

作者信息

Olah J, Feldmann H

出版信息

Nucleic Acids Res. 1980 May 10;8(9):1975-86. doi: 10.1093/nar/8.9.1975.

DOI:10.1093/nar/8.9.1975
PMID:6253952
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC324051/
Abstract

4 to 8 kb Hind III fragments of yeast DNA were cloned into pBR322. One of these clones (pY6m3) containing a single tRNA3Met gene has been characterized in detail. The DNA sequence of the structural gene is colinear with the tRNA sequence, which means that in this case no intervening sequence is present. The 5'-leader and 3'-trailer sequences have also been determined. The 5'-flanking region can be folded up into possible secondary structures.

摘要

酵母DNA的4至8kb Hind III片段被克隆到pBR322中。其中一个含有单个tRNA3Met基因的克隆(pY6m3)已被详细表征。结构基因的DNA序列与tRNA序列共线,这意味着在这种情况下不存在间隔序列。5'-前导序列和3'-尾随序列也已确定。5'-侧翼区域可以折叠成可能的二级结构。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c842/324051/32148c11b986/nar00426-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c842/324051/067aea0dc3e7/nar00426-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c842/324051/32148c11b986/nar00426-0095-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c842/324051/067aea0dc3e7/nar00426-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c842/324051/32148c11b986/nar00426-0095-a.jpg

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本文引用的文献

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The determination of the molecular weight of ribonucleic acid by polyacrylamide-gel electrophresis. The effects of changes in conformation.通过聚丙烯酰胺凝胶电泳测定核糖核酸的分子量。构象变化的影响。
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The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments.在分子杂交实验中使用体外经碘 - 125标记的RNA。
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Kinetic complexity of RNA molecules.RNA分子的动力学复杂性。
Mutations in the alpha-amanitin conserved domain of the largest subunit of yeast RNA polymerase III affect pausing, RNA cleavage and transcriptional transitions.
酵母RNA聚合酶III最大亚基的α-鹅膏蕈碱保守结构域中的突变会影响暂停、RNA切割和转录转换。
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Proc Natl Acad Sci U S A. 1984 May;81(10):2965-9. doi: 10.1073/pnas.81.10.2965.
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Isolation and structure of a yeast initiator tRNAmet gene.酵母起始甲硫氨酸转运RNA基因的分离与结构
Nucleic Acids Res. 1982 Feb 11;10(3):1093-6. doi: 10.1093/nar/10.3.1093.
8
Structural comparison of two yeast tRNA Glu 3 genes.两个酵母tRNA Glu 3基因的结构比较
Nucleic Acids Res. 1981 Jun 25;9(12):2961-70. doi: 10.1093/nar/9.12.2961.
9
Sequence of a yeast DNA fragment containing a chromosomal replicator and a tRNA Glu 3 gene.包含一个染色体复制起点和一个tRNA谷氨酸3基因的酵母DNA片段的序列。
Nucleic Acids Res. 1981 Jun 25;9(12):2949-59. doi: 10.1093/nar/9.12.2949.
10
Transfer RNA genes are genomic targets for de Novo transposition of the yeast retrotransposon Ty3.转运RNA基因是酵母逆转录转座子Ty3从头转座的基因组靶点。
Genetics. 1990 Dec;126(4):837-50. doi: 10.1093/genetics/126.4.837.
J Mol Biol. 1972 Jan 14;63(1):21-39. doi: 10.1016/0022-2836(72)90519-0.
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Transformation of Salmonella typhimurium by plasmid deoxyribonucleic acid.质粒脱氧核糖核酸对鼠伤寒沙门氏菌的转化
J Bacteriol. 1974 Sep;119(3):1072-4. doi: 10.1128/jb.119.3.1072-1074.1974.
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Restriction nucleases as probes of chromatin structure.作为染色质结构探针的限制性核酸内切酶。
Nature. 1975 Dec 4;258(5534):450-2. doi: 10.1038/258450a0.
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J Mol Biol. 1975 Nov 5;98(3):503-17. doi: 10.1016/s0022-2836(75)80083-0.
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FEBS Lett. 1975 Feb 1;50(2):185-9. doi: 10.1016/0014-5793(75)80485-6.