酵母非起始甲硫氨酸转运RNA基因的结构
Structure of a yeast non-initiating methionine-tRNA gene.
作者信息
Olah J, Feldmann H
出版信息
Nucleic Acids Res. 1980 May 10;8(9):1975-86. doi: 10.1093/nar/8.9.1975.
Abstract
4 to 8 kb Hind III fragments of yeast DNA were cloned into pBR322. One of these clones (pY6m3) containing a single tRNA3Met gene has been characterized in detail. The DNA sequence of the structural gene is colinear with the tRNA sequence, which means that in this case no intervening sequence is present. The 5'-leader and 3'-trailer sequences have also been determined. The 5'-flanking region can be folded up into possible secondary structures.
摘要
酵母DNA的4至8kb Hind III片段被克隆到pBR322中。其中一个含有单个tRNA3Met基因的克隆（pY6m3）已被详细表征。结构基因的DNA序列与tRNA序列共线，这意味着在这种情况下不存在间隔序列。5'-前导序列和3'-尾随序列也已确定。5'-侧翼区域可以折叠成可能的二级结构。
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