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采用高效液相色谱法和快原子轰击质谱法对人血浆中磷脂酰丝氨酸的分子种类进行部分表征。

Partial characterization of the molecular species of phosphatidylserine from human plasma by high-performance liquid chromatography and fast atom bombardment mass spectrometry.

作者信息

Chen S

机构信息

Department of Chemistry, University of Warwick, Coventry, UK.

出版信息

J Chromatogr B Biomed Appl. 1994 Nov 4;661(1):1-5. doi: 10.1016/0378-4347(94)00337-8.

DOI:10.1016/0378-4347(94)00337-8
PMID:7866537
Abstract

Phosphatidylserine from human plasma was purified and identified by amino normal-phase and octadecylsilyl reversed-phase high-performance liquid chromatography, respectively. The two major molecular species within the human plasma phosphatidylserine, qualitatively determined by negative-ion fast atom bombardment mass spectrometry, are 1-stearoyl-2-oleoyl-sn-glycero-3-phosphoserine and 1-stearoyl-2-arachidonyl-sn-glycero-3-phosphoserine.

摘要

分别通过氨基正相和十八烷基硅烷反相高效液相色谱法对人血浆中的磷脂酰丝氨酸进行了纯化和鉴定。通过负离子快原子轰击质谱法定性测定的人血浆磷脂酰丝氨酸中的两种主要分子种类为1-硬脂酰-2-油酰基-sn-甘油-3-磷酸丝氨酸和1-硬脂酰-2-花生四烯酰基-sn-甘油-3-磷酸丝氨酸。

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