Jones D J, Bjorksten A R
Anaesthesia Department, Royal Melbourne Hospital, Parkville, Vic., Australia.
J Chromatogr B Biomed Appl. 1994 Nov 4;661(1):165-7. doi: 10.1016/0378-4347(94)00333-5.
A high-performance liquid chromatographic method for the determination of the enantiomers of ketorolac in human plasma has been developed. Plasma samples containing ketorolac were acidified and extracted into diethyl ether. The ethereal extract was evaporated to dryness and the residue reconstituted in mobile phase before injection onto a Chiral-AGP column. The mobile phase was 2-propanol-20 mM potassium dihydrogenphosphate buffered to pH 7 (0.5:99.5, v/v). Detection was by ultraviolet absorbance at 320 nm. The detection limit was 5 ng/ml for each enantiomer. The method has been applied to determine the concentration of ketorolac enantiomers during an infusion of the racemic drug and has proven to be rapid and sensitive.
已开发出一种用于测定人血浆中酮咯酸对映体的高效液相色谱法。含酮咯酸的血浆样品经酸化后用乙醚萃取。将乙醚萃取液蒸发至干，残留物用流动相复溶后注入手性AGP柱。流动相为2 - 丙醇 - 20 mM磷酸二氢钾，用pH 7的缓冲液（0.5:99.5，v/v）。通过在320 nm处的紫外吸光度进行检测。每种对映体的检测限为5 ng/ml。该方法已用于测定消旋药物输注期间酮咯酸对映体的浓度，且已证明该方法快速且灵敏。
