Dubey Sunil K, Hemanth Jangala, Venkatesh K Chiranjeevi, Saha R N, Pasha S
Department of Pharmacy, Birla Institute of Technology & Science, Pilani, India.
Institute of Genomics and Integrative Biology, Mall Road, Delhi, India.
J Pharm Anal. 2012 Dec;2(6):462-465. doi: 10.1016/j.jpha.2012.07.006. Epub 2012 Jul 22.
A simple, specific, precise, sensitive and rapid reverse phase-HPLC method was developed for determination of ketorolac enantiomers, a potent nonnarcotic analgesic in pharmaceutical formulations. The method was developed on a chiral AGP column. Mobile phase was 0.1 M sodium phosphate buffer (pH 4.5): Isopropanol (98:2, v/v), at a flow rate of 1 mL/min with run time of 15 min. Ultraviolet detection was made at 322 nm. The linearity range was 0.02-10 μg/mL for each of the enantiomers. The mobile phase composition was systematically studied to find the optimum chromatographic conditions. Validation of the method under the conditions selected showed that it was selective and precise and that the detector response was linear function of ketorolac.
开发了一种简单、特异、精确、灵敏且快速的反相高效液相色谱法，用于测定酮咯酸对映体，酮咯酸是药物制剂中一种有效的非麻醉性镇痛药。该方法采用手性AGP柱。流动相为0.1M磷酸钠缓冲液（pH 4.5）：异丙醇（98:2，v/v），流速为1mL/min，运行时间为15分钟。在322nm处进行紫外检测。每种对映体的线性范围为0.02 - 10μg/mL。系统研究了流动相组成以找到最佳色谱条件。在所选择的条件下对该方法进行验证表明，它具有选择性和精确性，并且检测器响应是酮咯酸的线性函数。
