Hayball P J, Tamblyn J G, Holden Y, Wrobel J
Pharmacy Department, Repatriation General Hospital, Australia.
Chirality. 1993;5(1):31-5. doi: 10.1002/chir.530050107.
A high-performance liquid chromatographic (HPLC) analytical method is described for the quantification of the (R)- and (S)-enantiomers of ketorolac when present together in human plasma. The method involves derivatization with thionyl chloride/(S)-1-phenylethylamine and subsequent reversed-phase chromatography of the diastereomeric (S)-1-phenylethylamides of (R)- and (S)-ketorolac. The method is suitable for the analysis of large numbers of plasma samples and has been applied in this report to a pharmacokinetic study of ketorolac enantiomers upon intramuscular administration of racemic drug to a human subject. The limit of quantification for each enantiomer of ketorolac is 50 ng/ml (signal-to-noise ratio > 10).
本文描述了一种高效液相色谱（HPLC）分析方法，用于定量测定人血浆中同时存在的酮咯酸（R）-和（S）-对映体。该方法包括用亚硫酰氯/（S）-1-苯乙胺进行衍生化，随后对（R）-和（S）-酮咯酸的非对映体（S）-1-苯乙酰胺进行反相色谱分析。该方法适用于大量血浆样品的分析，本报告已将其应用于对一名人类受试者肌肉注射外消旋药物后酮咯酸对映体的药代动力学研究。酮咯酸各对映体的定量限为50 ng/ml（信噪比>10）。
