Lema O E, Carter J Y, Arube P A, Munafu C G, Wangai M W, Rees P H
Clinical Department, African Medical and Research Foundation (AMREF), Nairobi, Kenya.
Bull World Health Organ. 1994;72(6):937-41.
In many health facilities in east Africa, haemoglobin estimation is performed using visual colour comparison methods. Efforts to establish colorimetric methods face numerous constraints, including the unavailability of standards for quality control. In contrast, the alkaline haematin D-575 method for haemoglobin estimation is a colorimetric method that uses primary standards prepared from pure, crystalline chlorohaemin. There is no significant difference in the accuracy of the alkaline haematin D-575 method and that of the reference haemiglobincyanide method (P > 0.05), and the response of the alkaline haematin D-575 method is linear for serially diluted blood samples over the haemoglobin concentration range 19.6-3.3 g/dl (r = 0.994, y = 1.01 x - 0.3). The method has a precision of +/-0.3 g/dl (coefficient of variation = (1.8%) for whole blood, and is suitable for use with fixed-wavelength haemglobinometers (lambda = 565 nm) or with colorimeters at lambda = 580 nm. Stable quality control standards could be prepared at provincial, zonal, or reference laboratories and distributed regularly to outlying laboratories.
在东非的许多医疗机构中，血红蛋白估算是通过目视颜色比较法进行的。建立比色法的努力面临诸多限制，包括缺乏质量控制标准。相比之下，用于血红蛋白估算的碱性高铁血红素D-575法是一种比色法，它使用由纯结晶氯高铁血红素制备的一级标准品。碱性高铁血红素D-575法与参比氰化高铁血红蛋白法在准确性上无显著差异（P>0.05），并且在血红蛋白浓度范围为19.6 - 3.3 g/dl的系列稀释血样中，碱性高铁血红素D-575法的响应呈线性（r = 0.994，y = 1.01x - 0.3）。该方法的精密度为全血±0.3 g/dl（变异系数=(1.8%)，适用于固定波长血红蛋白计（λ = 565 nm）或波长为λ = 580 nm的比色计。稳定的质量控制标准品可在省级、区域或参比实验室制备，并定期分发给偏远实验室。
