血红蛋白比色计的评估及与血细胞比容血红蛋白测定法的比较。
Evaluation of the Haemoglobin Colour Scale and comparison with the HemoCue haemoglobin assay.
作者信息
Paddle J J
机构信息
Department of Anaesthesia, Royal Cornwall Hospital, England.
出版信息
Bull World Health Organ. 2002;80(10):813-6. Epub 2002 Nov 28.
OBJECTIVE
To evaluate the Haemoglobin Colour Scale developed by WHO for estimating haemoglobin concentration and to compare the results obtained using it and the HemoCue assay with those determined using a reference method, the Technicon H3 analyser.
METHODS
The Colour Scale and HemoCue assay were used to test 408 blood samples. Subsequently, Bland-Altman plots were determined and the proximity of the test results to those obtained using the reference method was determined.
FINDINGS
The mean difference between the Haemoglobin Colour Scale and the reference method was 0.19 g/dl (95% confidence interval: 3.50 g/dl below to 3.11 g/dl above); the corresponding value for the HemoCue assay was 0.50 g/dl (1.16 g/dl below to 0.16 g/dl above). Only 46.08% of the results obtained by means of the Colour Scale were within 1.0 g/dl of the reference method, whereas 95.34% of the HemoCue results fell within this limit; 22.79% of the Colour Scale results but none of the HemoCue results lay more than 2.0 g/dl from the reference method.
CONCLUSION
The Haemoglobin Colour Scale test is too inaccurate for general use, particularly if devices such as the HemoCue are available.
目的
评估世界卫生组织开发的用于估计血红蛋白浓度的血红蛋白比色卡，并将使用该比色卡和血红蛋白检测系统（HemoCue）获得的结果与使用参考方法Technicon H3分析仪测定的结果进行比较。
方法
使用比色卡和HemoCue检测系统对408份血样进行检测。随后，绘制布兰德-奥特曼图，并确定检测结果与使用参考方法获得的结果的接近程度。
结果
血红蛋白比色卡与参考方法之间的平均差异为0.19 g/dl（95%置信区间：低于3.50 g/dl至高于3.11 g/dl）；HemoCue检测系统的相应值为0.50 g/dl（低于1.16 g/dl至高于0.16 g/dl）。通过比色卡获得的结果中只有46.08%在参考方法的1.0 g/dl范围内，而HemoCue检测结果的95.34%落在该范围内；比色卡结果的22.79%但HemoCue检测结果无一超过参考方法2.0 g/dl。
结论
血红蛋白比色卡检测过于不准确，不适合一般使用，特别是在有HemoCue等设备的情况下。
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