Byrne A M, Kukor J J, Olsen R H
Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620, USA.
Gene. 1995 Feb 27;154(1):65-70. doi: 10.1016/0378-1119(94)00844-i.
The nucleotide (nt) sequence and gene organization of the locus encoding the initial step of the toluene-3-monooxygenase (Tbu) pathway from Pseudomonas pickettii PKO1 has been determined. This is the first reported nt sequence for a toluene monooxygenase which hydroxylates the C-3 position of toluene. Six tightly assembled structural genes encoding several Tbu were identified and were designated tbuA1, tbuU, tbuB, tbuV, tbuA2 and tbuC. Comparison of the deduced amino acid (aa) sequences of each open reading frame (ORF) with translated sequences from the GenBank database revealed significant overall homology to peptides from the toluene-4-monooxygenase (Tmo) from Pseudomonas mendocina KR1, the multicomponent phenol hydroxylase (Dmp) from Pseudomonas sp. strain CF600 and the methane monooxygenases (Mmo) from both Methylococcus capsulatus (Bath) and Methylosinus trichosporium OB3b. Similarities in both size and aa sequence between the peptides from these multicomponent oxygenases and the putative peptides from Tbu suggested roles for the TbuA1, TbuB, TbuV, TbuA2 and TbuC proteins.
已确定来自皮氏假单胞菌PKO1的甲苯-3-单加氧酶（Tbu）途径起始步骤编码基因座的核苷酸（nt）序列和基因组织。这是首次报道的使甲苯C-3位羟基化的甲苯单加氧酶的nt序列。鉴定出六个紧密组装的编码几种Tbu的结构基因，并将其命名为tbuA1、tbuU、tbuB、tbuV、tbuA2和tbuC。将每个开放阅读框（ORF）推导的氨基酸（aa）序列与来自GenBank数据库的翻译序列进行比较，发现与来自门多萨假单胞菌KR1的甲苯-4-单加氧酶（Tmo）、假单胞菌属CF600菌株的多组分苯酚羟化酶（Dmp）以及荚膜甲基球菌（巴斯）和 trichosporium OB3b甲基弯曲菌的甲烷单加氧酶（Mmo）的肽具有显著的总体同源性。这些多组分加氧酶的肽与Tbu推定肽在大小和aa序列上的相似性表明了TbuA1、TbuB、TbuV、TbuA2和TbuC蛋白的作用。
