Verspaget H W, Clemmensen I, Ganesh S, Christensen L, Sier C F, Griffioen G, Lamers C B
Department of Gastroenterology and Hepatology, University Hospital Leiden, The Netherlands.
Histopathology. 1994 Nov;25(5):463-7. doi: 10.1111/j.1365-2559.1994.tb00008.x.
The expression of tetranectin in colonic neoplasia was evaluated by determining the tissue distribution by immunohistological analysis of tissue sections and the antigen levels in tissue homogenates and plasma. In normal colonic mucosa tetranectin staining was predominantly found in the goblet cells whereas in adenocarcinomas this staining was confined to the tumour stroma. Colonic adenomas, benign precursors of adenocarcinomas, showed fewer tetranectin positive goblet cells and in some cases showed tetranectin expression in the stroma. Within the tissue homogenates no differences were found in the tetranectin levels between normal mucosa, adenomas and carcinomas. Patients with colonic cancer were found to have significantly decreased plasma tetranectin levels compared to healthy controls. Thus, colonic neoplasia is associated with a change in the tissue distribution of tetranectin, without an obvious change in the tissue level, and a low plasma tetranectin level.
通过对组织切片进行免疫组织学分析确定组织分布以及测定组织匀浆和血浆中的抗原水平，来评估结肠肿瘤中纤连蛋白的表达。在正常结肠黏膜中，纤连蛋白染色主要见于杯状细胞，而在腺癌中，这种染色局限于肿瘤基质。结肠腺瘤是腺癌的良性前体，其杯状细胞中纤连蛋白阳性的细胞较少，在某些情况下，基质中也有纤连蛋白表达。在组织匀浆中，正常黏膜、腺瘤和癌之间的纤连蛋白水平没有差异。与健康对照相比，结肠癌患者的血浆纤连蛋白水平显著降低。因此，结肠肿瘤与纤连蛋白的组织分布变化相关，而组织水平无明显变化，且血浆纤连蛋白水平较低。
