通过对nifH基因序列进行分子进化分析，在水稻根中检测到显著的固氮细菌多样性。
Remarkable N2-fixing bacterial diversity detected in rice roots by molecular evolutionary analysis of nifH gene sequences.
作者信息
Ueda T, Suga Y, Yahiro N, Matsuguchi T
机构信息
Department of Agricultural Chemistry, Kyushu University, Fukuoka, Japan.
出版信息
J Bacteriol. 1995 Mar;177(5):1414-7. doi: 10.1128/jb.177.5.1414-1417.1995.
Abstract
To demonstrate the extent of phylogenetic diversity of diazotrophic bacteria associated with rice roots, we characterized phylogenetically 23 nifH gene sequences obtained by PCR amplification of mixed organism DNA extracted directly from rice roots without culturing the organisms. The analyses document the presence of eight novel NifH types, which appear to be a variety of significant components of the diazotrophic community, dominated mainly by proteobacteria.
摘要
为了证明与水稻根系相关的固氮细菌的系统发育多样性程度，我们对通过PCR扩增直接从水稻根系提取的混合微生物DNA获得的23个nifH基因序列进行了系统发育特征分析，而未对这些微生物进行培养。分析结果表明存在8种新型NifH类型，它们似乎是固氮群落的各种重要组成部分，主要由变形菌门主导。
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