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尽管经过了几十年的施肥，植物根系仍会影响温带草原土壤中的自由生活固氮菌群落。

Plant roots affect free-living diazotroph communities in temperate grassland soils despite decades of fertilization.

机构信息

Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, University of Vienna, Vienna, Austria.

Institute of Soil Biology and Biogeochemistry, Biology Centre CAS, České Budějovice, Czechia.

出版信息

Commun Biol. 2024 Jul 11;7(1):846. doi: 10.1038/s42003-024-06522-w.

DOI:10.1038/s42003-024-06522-w
PMID:38987659
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11237082/
Abstract

Fixation of atmospheric N by free-living diazotrophs accounts for an important proportion of nitrogen naturally introduced to temperate grasslands. The effect of plants or fertilization on the general microbial community has been extensively studied, yet an understanding of the potential combinatorial effects on the community structure and activity of free-living diazotrophs is lacking. In this study we provide a multilevel assessment of the single and interactive effects of different long-term fertilization treatments, plant species and vicinity to roots on the free-living diazotroph community in relation to the general microbial community in grassland soils. We sequenced the dinitrogenase reductase (nifH) and the 16S rRNA genes of bulk soil and root-associated compartments (rhizosphere soil, rhizoplane and root) of two grass species (Arrhenatherum elatius and Anthoxanthum odoratum) and two herb species (Galium album and Plantago lanceolata) growing in Austrian grassland soils treated with different fertilizers (N, P, NPK) since 1960. Overall, fertilization has the strongest effect on the diazotroph and general microbial community structure, however with vicinity to the root, the plant effect increases. Despite the long-term fertilization, plants strongly influence the diazotroph communities emphasizing the complexity of soil microbial communities' responses to changing nutrient conditions in temperate grasslands.

摘要

自生固氮菌对大气氮的固定占温带草原自然引入氮的重要比例。植物或施肥对一般微生物群落的影响已经得到了广泛的研究，但对于它们对自生固氮菌群落结构和活性的潜在组合效应的理解却很缺乏。在这项研究中，我们对不同长期施肥处理、植物物种和靠近根系对与草地土壤中一般微生物群落相关的自生固氮菌群落的单一和交互影响进行了多层次评估。我们对两种草本植物（鹅观草和黄花茅）和两种草本植物（繁缕和车前草）的根际土壤（根际土壤、根面和根）和根相关区室（根际土壤、根面和根）的二氮还原酶（nifH）和 16S rRNA 基因进行了测序，这些植物生长在奥地利草地土壤中，自 1960 年以来，这些土壤分别接受了不同的肥料（N、P、NPK）处理。总的来说，施肥对固氮菌和一般微生物群落结构的影响最强，但靠近根系，植物的影响增加。尽管长期施肥，但植物对固氮菌群落有很强的影响，这强调了温带草原土壤微生物群落对不断变化的养分条件的响应的复杂性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/d1517bd835de/42003_2024_6522_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/b285538d824d/42003_2024_6522_Fig1_HTML.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/262b9075de85/42003_2024_6522_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/dbd21bed0f66/42003_2024_6522_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/fe13a96ac9b4/42003_2024_6522_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/d1517bd835de/42003_2024_6522_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/b285538d824d/42003_2024_6522_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/e3f72a92475f/42003_2024_6522_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/5f44fee99914/42003_2024_6522_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/262b9075de85/42003_2024_6522_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/dbd21bed0f66/42003_2024_6522_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/fe13a96ac9b4/42003_2024_6522_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6091/11237082/d1517bd835de/42003_2024_6522_Fig7_HTML.jpg

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