在间接酶联免疫吸附测定中使用重组抗原检测牛抗山羊痘病毒抗体。
Use of a recombinant antigen in an indirect ELISA for detecting bovine antibody to capripoxvirus.
作者信息
Carn V M, Kitching R P, Hammond J M, Chand P
机构信息
AFRC Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, UK.
出版信息
J Virol Methods. 1994 Oct;49(3):285-94. doi: 10.1016/0166-0934(94)90143-0.
The gene coding for the capripoxvirus structural protein P32 was cloned, expressed in Escherichia coli as a fusion protein with glutathione-S-transferase, and purified on glutathione Sepharose. An indirect enzyme linked immunosorbent assay (ELISA) using this antigen was developed to screen bovine sera for antibodies to capripoxvirus. Sequential serum samples from experimentally infected animals tested by ELISA and by virus neutralisation test (VNT) showed that the ELISA was more sensitive and detected antibodies to capripoxvirus earlier post-infection than the VNT.
编码山羊痘病毒结构蛋白P32的基因被克隆出来，在大肠杆菌中作为与谷胱甘肽-S-转移酶的融合蛋白进行表达，并在谷胱甘肽琼脂糖凝胶上进行纯化。利用该抗原开发了一种间接酶联免疫吸附测定（ELISA），用于筛查牛血清中的山羊痘病毒抗体。通过ELISA和病毒中和试验（VNT）对实验感染动物的连续血清样本进行检测，结果表明ELISA比VNT更敏感，并且在感染后更早地检测到山羊痘病毒抗体。
Zotero 插件