Foucault P, Drosdowsky M A, Carreau S
Laboratoire de Biochimie, CHU Côte de Nacre, France.
Hum Reprod. 1994 Nov;9(11):2062-8. doi: 10.1093/oxfordjournals.humrep.a138394.
In human Sertoli cell preparations obtained from healthy men (mean age 31.8 +/- 6.8 years; n = 6), we have measured the productions of lactate, 17 beta-oestradiol, transferrin and inhibin between day 4 and day 5 after plating, either in the presence or absence (hypotonic treatment of plated cells on day 2) of germ cells. The results, expressed per 10(6) of cells plated/24 h, showed that lactate production was unchanged, whether or not germ cells were present. However, if we calculated the lactate production per mg protein/24 h, the lactate output was decreased (30-60%) in the presence of germ cells. Whatever the mode of expression, Sertoli cell 17 beta-oestradiol synthesis was diminished 1.5-fold in the presence of germ cells. Conversely, the transferrin output was increased 3.2-fold in non-treated Sertoli cell preparations when compared to the hypotonic-treated plates. A similar observation was recorded for the in-vitro production of inhibin by Sertoli cells, which was enhanced 1.4-fold when germ cells were present. These results, together with a likely potentializing role of germ cells on follicle stimulating hormone control of Sertoli cell function, strongly suggest that germ cells exert both stimulatory and inhibitory effects in regulating human Sertoli cell function through either direct contact and/or via secreted factors.
在从健康男性（平均年龄31.8±6.8岁；n = 6）获取的人支持细胞制剂中，我们测定了接种后第4天至第5天之间，在有或没有生殖细胞存在的情况下（第2天对接种细胞进行低渗处理）乳酸、17β-雌二醇、转铁蛋白和抑制素的生成量。结果以每接种10⁶个细胞/24小时表示，显示无论有无生殖细胞存在，乳酸生成量均无变化。然而，如果我们计算每毫克蛋白质/24小时的乳酸生成量，在有生殖细胞存在的情况下，乳酸产量会降低（30 - 60%）。无论表达方式如何，在有生殖细胞存在的情况下，支持细胞17β-雌二醇的合成减少了1.5倍。相反，与低渗处理的平板相比，未处理的支持细胞制剂中转铁蛋白的产量增加了3.2倍。对于支持细胞体外抑制素的生成也有类似观察结果，当有生殖细胞存在时，其产量提高了1.4倍。这些结果，连同生殖细胞对支持细胞功能的促卵泡生成素控制可能具有的潜在增强作用，强烈表明生殖细胞通过直接接触和/或分泌因子，在调节人支持细胞功能中发挥刺激和抑制作用。
