Hopfer R L, Gröschel D
J Clin Microbiol. 1976 Aug;2(2):96-8.
Cryptococcus neoformans colonies can be identified within 6 h using paper disks containing caffeic acid and ferric citrate. Indentification is based on the development of a dark brown pigment. Saprophytic Cryptococcus species and common clinically isolated yeasts do not develop the brown color. The concentration of the reagents and the method of storage of the impregnated paper disks are critical for the rapid and specific development of the pigment.
新型隐球菌菌落可在6小时内使用含有咖啡酸和柠檬酸铁的纸片进行鉴定。鉴定基于深棕色色素的形成。腐生隐球菌物种和常见的临床分离酵母不会形成棕色。试剂的浓度和浸渍纸片的储存方法对于色素的快速和特异性形成至关重要。
