Geary S J, Forsyth M H, Aboul Saoud S, Wang G, Berg D E, Berg C M
Department of Pathobiology, University of Connecticut 06269-3089.
Mol Cell Probes. 1994 Aug;8(4):311-6. doi: 10.1006/mcpr.1994.1042.
We demonstrate here that the arbitrary primer polymerase-chain-reaction-based DNA fingerprinting method (also termed random amplified polymorphic DNA or RAPD) can be used to distinguish among strains of the avian pathogen Mycoplasma gallisepticum. Ten base oligonucleotide primers were used individually to prime DNA synthesis from genomic DNAs. Strain-specific arrays of DNA fragments were generated, which allowed us to identify and group isolates. Isolates of M. synoviae, M. gallinarum and M. iners yielded arrays of DNA fragments that differed markedly from those generated from the M. gallisepticum isolates using the same arbitrary primers. These results show that the RAPD fingerprinting method distinguishes genetically different strains of M. gallisepticum and indicates that it should be valuable for monitoring transmission of this pathogen.
我们在此证明，基于任意引物聚合酶链反应的DNA指纹图谱法（也称为随机扩增多态性DNA或RAPD）可用于区分禽病原体鸡毒支原体的不同菌株。使用十个碱基的寡核苷酸引物分别从基因组DNA引发DNA合成。产生了菌株特异性的DNA片段阵列，这使我们能够识别和分组分离株。滑液支原体、鸡支原体和惰性支原体的分离株产生的DNA片段阵列与使用相同任意引物从鸡毒支原体分离株产生的阵列明显不同。这些结果表明，RAPD指纹图谱法可区分鸡毒支原体遗传上不同的菌株，并表明它对于监测这种病原体的传播应该是有价值的。
