鸡败血支原体S6基因组的物理图谱及选定基因的定位
Physical mapping of the Mycoplasma gallisepticum S6 genome with localization of selected genes.
作者信息
Gorton T S, Goh M S, Geary S J
机构信息
Department of Pathobiology, University of Connecticut, Storrs 06269-3089.
出版信息
J Bacteriol. 1995 Jan;177(1):259-63. doi: 10.1128/jb.177.1.259-263.1995.
Abstract
We report the construction of a physical map of the Mycoplasma gallisepticum S6 genome by field-inversion gel electrophoresis of DNA fragments generated by digestion of genomic DNA with rare-cutting restriction endonucleases. The size of the M. gallisepticum S6 genome was calculated to be approximately 1,054 kb. The loci of several genes have been assigned to the map by Southern hybridization utilizing specific gene probes.
摘要
我们报道了通过用稀有切割限制性内切酶消化基因组 DNA 产生的 DNA 片段进行场反转凝胶电泳构建鸡毒支原体 S6 基因组物理图谱的过程。计算得出鸡毒支原体 S6 基因组大小约为 1054 kb。利用特异性基因探针通过 Southern 杂交已将几个基因的位点定位到该图谱上。
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