Tanojo H, Bouwstra J A, Junginger H E, Boddé H E
Division of Pharmaceutical Technology, Leiden/Amsterdam Center for Drug Research, Leiden University, The Netherlands.
Pharm Res. 1994 Nov;11(11):1610-6. doi: 10.1023/a:1018965906027.
The thermal behaviour of human stratum corneum was studied using differential thermal analysis within the temperature range of -130 degrees C to 120 degrees C. Aside from thermal transitions at around 40 degrees C, 70 degrees C, 85 degrees C and 100 degrees C, which have been reported before, a particular transition below 0 degree C (subzero), at approx. -9 degrees C (264 K), was noticed. This transition was present in the analysis curves of dehydrated as well as hydrated stratum corneum sheets and could be distinguished from the water peak found only in hydrated stratum corneum samples. To further characterize this transition, thermal analysis was performed on stratum corneum sheets: (i) after lipid extraction, (ii) after pretreatment of propylene glycol and (iii) after pretreatment of oleic acid/propylene glycol solution. From the results, it was concluded that the subzero transition (-9 degrees C) belongs to low melting lipid components of stratum corneum.
在-130℃至120℃的温度范围内，使用差示热分析法研究了人体角质层的热行为。除了之前报道的在约40℃、70℃、85℃和100℃处的热转变外，还注意到在0℃以下（零下）约-9℃（264K）处有一个特殊的转变。这种转变存在于脱水和水合角质层薄片的分析曲线中，并且可以与仅在水合角质层样品中发现的水峰区分开来。为了进一步表征这种转变，对角质层薄片进行了热分析：（i）脂质提取后，（ii）丙二醇预处理后，以及（iii）油酸/丙二醇溶液预处理后。从结果得出结论，零下转变（-9℃）属于角质层的低熔点脂质成分。
