Golden G M, Guzek D B, Harris R R, McKie J E, Potts R O
J Invest Dermatol. 1986 Mar;86(3):255-9. doi: 10.1111/1523-1747.ep12285373.
The techniques of differential scanning calorimetry (DSC) and thermal perturbation infrared (IR) spectrometry were used to investigate thermal transitions in intact, fractionated, and lipid-extracted human stratum corneum. The DSC results show 3 major and one minor thermal transition in the range of 30-120 degrees C. Of particular interest to this study are 2 transitions seen near 65 degrees and 75 degrees C in intact stratum corneum and a stratum corneum membrane preparation, but absent from lipid-extracted samples. Results of IR spectrometry show that thermally induced spectral changes related to enhanced motion of the lipid acyl chains also occur in the region of about 60-80 degrees C. The combined DSC and IR results show that the thermal transitions near 65 degrees and 75 degrees C depend on water concentration in a manner identical to that seen for a variety of lipid-water systems. Taken together, these results suggest that thermal transitions occurring near 65 degrees and 85 degrees C involve increased acyl chain motion of stratum corneum lipids.
采用差示扫描量热法（DSC）和热扰动红外（IR）光谱技术，研究完整的、分级分离的以及脂质提取后的人体角质层中的热转变。DSC结果显示，在30 - 120摄氏度范围内有3个主要热转变和1个次要热转变。本研究特别感兴趣的是，在完整角质层和角质层膜制剂中，在65摄氏度和75摄氏度附近出现了2个转变，但在脂质提取的样品中未出现。红外光谱结果表明，与脂质酰基链运动增强相关的热诱导光谱变化也发生在约60 - 80摄氏度的区域。DSC和IR的综合结果表明，65摄氏度和75摄氏度附近的热转变取决于水浓度，其方式与多种脂质 - 水系统中观察到的相同。综上所述，这些结果表明，在65摄氏度和85摄氏度附近发生的热转变涉及角质层脂质酰基链运动的增加。
