Wang Q, Pantzar N, Jeppsson B, Weström B R, Karlsson B W
Dept. of Animal Physiology, Lund University, Sweden.
Scand J Gastroenterol. 1994 Nov;29(11):1001-8. doi: 10.3109/00365529409094877.
The intestinal barrier properties are impaired during inflammation and sepsis, but the mechanisms behind this are unknown and were therefore investigated during experimental sepsis in rats.
The different-sized intestinal absorption markers 51Cr-labeled ethylenediaminetetraacetic acid (EDTA) and ovalbumin were gavaged to rats made septic by intra-abdominal bacterial implantation and to sham-operated rats. Regional tissue permeability was measured in diffusion chambers, and intestinal transit was evaluated by intestinal accumulation of gavaged 51Cr-EDTA.
In comparison with the sham-operated rats, septic rats had higher 51Cr-EDTA levels in blood and urine and showed a prolonged intestinal transit. Septic rats also had a lower tissue permeability to both markers in the small intestines but higher permeability to ovalbumin in the colon. Rats receiving morphine to decrease intestinal motility showed similar changes, with a decreased intestinal transit and increased marker absorption.
The results suggest that the increased intestinal absorption during sepsis was due to regional permeability changes and prolonged intestinal transit.
在炎症和脓毒症期间，肠道屏障功能受损，但其背后的机制尚不清楚，因此在大鼠实验性脓毒症期间对其进行了研究。
将不同大小的肠道吸收标志物51Cr标记的乙二胺四乙酸（EDTA）和卵清蛋白灌胃给通过腹腔内细菌植入制成脓毒症的大鼠以及假手术大鼠。在扩散室中测量局部组织通透性，并通过灌胃的51Cr-EDTA在肠道中的蓄积来评估肠道转运。
与假手术大鼠相比，脓毒症大鼠血液和尿液中的51Cr-EDTA水平更高，肠道转运时间延长。脓毒症大鼠对小肠中两种标志物的组织通透性也较低，但对结肠中卵清蛋白的通透性较高。接受吗啡以降低肠道蠕动的大鼠表现出类似的变化，肠道转运减少，标志物吸收增加。
结果表明，脓毒症期间肠道吸收增加是由于局部通透性变化和肠道转运时间延长所致。
