Takano M, Ohara N, Mizuno A, Yamada T
Nagasaki University School of Dentistry, Japan.
Scand J Immunol. 1994 Aug;40(2):165-70. doi: 10.1111/j.1365-3083.1994.tb03446.x.
The gene for the extracellular alpha antigen of Mycobacterium scrofulaceum (S-alpha) was cloned by using the alpha antigen gene fragments of Mycobacterium bovis BCG as probes. The complete nucleotide sequence was determined. The gene was expressed in Escherichia coli. The gene encodes 330 amino acids, including 40 amino acids for the signal peptide, followed by 290 amino acids for the mature protein. The deduced amino acid sequences were highly homologous to the alpha antigen of the species of other mycobacteria. Interestingly, the alpha antigens of MAIS complex (Mycobacterium avium-Mycobacterium intracellulare-M. scrofulaceum) were closely homologous even at the C-terminal regions which were variable among those of M. bovis BCG, Mycobacterium leprae and Mycobacterium kansasii.
用牛分枝杆菌卡介苗（BCG）的α抗原基因片段作探针，克隆了瘰疬分枝杆菌细胞外α抗原基因（S-α）。测定了其完整的核苷酸序列。该基因在大肠杆菌中表达。该基因编码330个氨基酸，其中包括40个氨基酸的信号肽，随后是290个氨基酸的成熟蛋白。推导的氨基酸序列与其他分枝杆菌属的α抗原高度同源。有趣的是，MAIS复合体（鸟分枝杆菌-胞内分枝杆菌-瘰疬分枝杆菌）的α抗原即使在C末端区域也高度同源，而该区域在BCG、麻风分枝杆菌和堪萨斯分枝杆菌中是可变的。
