Revol A, Badonnel Y, Carreau S, Castanier M, Collet C, Delvigne L, Denis I, Guilloteau D, Moatti J P, Patricot M C
Laboratoire de biochimie, centre hospitalier Lyon-Sud, France.
Ann Biol Clin (Paris). 1994;52(9):671-80.
The discrepancy between results obtained using different anti-hLH antibodies available on the market led us (in the context of a contract with the CNAMTS) to compare the results of hLH immunoassays (eight different reagents) with those of hLH bioassays based on the measurement of the steroidal activity of this hormone on mouse, rat or pig testicular preparations (five methodologies). Twenty-nine samples of serum or human serum pools were tested. The values resulting from these tests reveal differences linked to the nature of the antibodies or to the methodological conditions used for the testicular preparations. Some antibodies seem unable to identify some hLH forms which, however, can be detected by biological methods.
市场上不同抗人促黄体生成素（hLH）抗体所获结果之间的差异，促使我们（在与法国社会保障医疗事务局签订的合同背景下）将hLH免疫测定（八种不同试剂）的结果与基于该激素对小鼠、大鼠或猪睾丸制剂甾体活性测量的hLH生物测定结果（五种方法）进行比较。对29份血清样本或人血清混合样本进行了检测。这些检测所得结果揭示了与抗体性质或睾丸制剂所用方法条件相关的差异。一些抗体似乎无法识别某些hLH形式，然而这些形式可通过生物学方法检测到。
