Revol A, Carreau S, Castanier M, Collet C, Denis I, Guilloteau D, Moatti J P, Patricot M C, Roger M
Laboratoire de biochimie, Centre hospitalier Lyon-Sud, Pierre-Bénite.
Ann Biol Clin (Paris). 1997 Mar-Apr;55(2):123-8.
This report describes the results of a multicentric study of biological methods for luteinizing hormone (hLH). The production of testosterone by animal Leydig cells is used by five laboratories with different methodologies including rat, mouse and pig cells. Dose-response curves of testosterone production, quality criteria and results on a physiological population of men and women, are reported and discussed. It is concluded that the bioactive determination of hLH must be considered as a reference method used in discordances between clinic and immunological methods.
本报告描述了一项关于促黄体生成素（hLH）生物学检测方法的多中心研究结果。五个实验室采用不同方法，利用动物睾丸间质细胞（包括大鼠、小鼠和猪的细胞）来检测睾酮的生成。报告并讨论了睾酮生成的剂量反应曲线、质量标准以及在男性和女性生理群体中的检测结果。得出的结论是，hLH的生物活性测定必须被视为在临床方法与免疫方法出现不一致时所使用的参考方法。
