Kao S F, Kuo H L, Lee Y C, Chiang H C, Lin Y S
Department of Microbiology and Immunology, National Cheng Kung University Medical College, Tainan, Taiwan, Republic of China.
Anticancer Res. 1994 Nov-Dec;14(6B):2439-43.
Alsophila spinulosa (Cyatheaceae) has been used in Asia as a herbal medicine. In this study, the immunomodulatory effects of Alsophila spinulosa dried stems water extract fraction VII (AS-VII) were studied in vitro using an animal model. The results showed that AS-VII stimulated splenocyte proliferation in both BALB/c and C3H/HeJ mice. By cell fractionation analysis, the results showed that the mitogenic effect of AS-VII was predominantly on B cell population. The antibody response was also augmented by AS-VII in BALB/c and C3H/HeJ mice. The augmentation of immune responses in C3H/HeJ, the lipopolysaccharide (LPS)-hyporesponsive mouse strain, indicated that the activity was not simply due to the LPS contamination which may be present in AS-VII preparation. Furthermore, AS-VII caused an increase in cytotoxic T cell activity in a mixed lymphocyte reaction culture. These data indicate that AS-VII possesses the capability of augmenting both humoral and cellular immune responses.
桫椤（桫椤科）在亚洲被用作草药。在本研究中，使用动物模型在体外研究了桫椤干燥茎水提取物组分VII（AS-VII）的免疫调节作用。结果表明，AS-VII刺激了BALB/c和C3H/HeJ小鼠的脾细胞增殖。通过细胞分级分析，结果表明AS-VII的促有丝分裂作用主要作用于B细胞群体。AS-VII也增强了BALB/c和C3H/HeJ小鼠的抗体反应。在C3H/HeJ（脂多糖（LPS）低反应性小鼠品系）中免疫反应的增强表明，该活性并非简单地归因于AS-VII制剂中可能存在的LPS污染。此外，AS-VII在混合淋巴细胞反应培养中导致细胞毒性T细胞活性增加。这些数据表明，AS-VII具有增强体液免疫和细胞免疫反应的能力。
