Muta H, Iguchi H, Hayashi I, Wakasugi H, Misawa T, Niwata H, Kono A
Department of Gastroenterology, National Kyushu Cancer Center, Kukuoka, Japan.
Hum Cell. 1994 Sep;7(3):172-5.
A human colon cancer-derived cell line, KC-1, was established from the surgical specimen of mucinous adenocarcinoma of the colon. The cells grew as monolayers, showing formation of irregular aggregation of cells and pleomorphic nuclei. The doubling time in vitro was 56.6 hours. The cells produced CEA, CA19-9 and sialyl SSEA-1(SLX). Chromosome numbers were distributed between 79 and 83 with many structural abnormalities. A point mutation of the Ki-ras gene in codon 61 (CAA-->CAT) was found. The cells have been subcultured 13 times during these three years. This cell line can be useful for investigations of colon cancer.
一种人结肠癌衍生细胞系KC-1，是从结肠黏液腺癌手术标本中建立的。细胞呈单层生长，表现出细胞不规则聚集和核多形性。体外倍增时间为56.6小时。这些细胞产生癌胚抗原（CEA）、糖类抗原19-9（CA19-9）和唾液酸化阶段特异性胚胎抗原-1（SLX）。染色体数目分布在79至83之间，有许多结构异常。发现Ki-ras基因第61密码子存在点突变（CAA→CAT）。在这三年中，这些细胞已传代培养13次。该细胞系可用于结肠癌的研究。
