Davies W, Høyheim B, Chaput B, Archibald A L, Frelat G
Norwegian College of Veterinary Medicine, Department of Biochemistry, Physiology and Nutrition, Oslo.
Mamm Genome. 1994 Nov;5(11):707-11. doi: 10.1007/BF00426077.
Porcine flow-sorted Chromosome (Chr) 13 was PCR amplified with primers based on porcine short interspersed element (SINE) sequences. The product was cloned, gridded in microtiter plates, and screened with a [GT]10 oligonucleotide which gave 45 positive clones. Sequencing of these clones showed that 36 were unique, and 26 [GT]n microsatellites were characterized. Six other simple repeat sequences, the majority of which were associated with the 3' end of the SINE sequence, were also detected. Twenty-one primers sets were selected, and 13 of these detected useful polymorphisms in the grandparents (n = 26) of the European porcine mapping collaboration (PiGMaP) reference families. These 13 markers were mapped in the "PiGMaP" reference families, and a two-point linkage analysis was performed. The Lod scores indicated that three of the markers were not linked and the remaining 11 formed two linkage groups of two and nine markers respectively. The larger linkage group was also linked to the transferrin locus, permitting assignment of nine markers to porcine Chr 13.
利用基于猪短散在元件（SINE）序列的引物对猪经流式细胞术分选的13号染色体（Chr）进行PCR扩增。将产物克隆，点样于微量滴定板中，并用[GT]10寡核苷酸进行筛选，得到45个阳性克隆。对这些克隆进行测序表明，其中36个是独特的，并对26个[GT]n微卫星进行了特征分析。还检测到其他6个简单重复序列，其中大多数与SINE序列的3'端相关。选择了21对引物，其中13对在欧洲猪基因定位协作组（PiGMaP）参考家系的祖代（n = 26）中检测到有用的多态性。将这13个标记定位到“PiGMaP”参考家系中，并进行两点连锁分析。连锁值表明，其中3个标记不连锁，其余11个分别形成了由2个和9个标记组成的两个连锁群。较大的连锁群也与转铁蛋白基因座连锁，从而将9个标记定位到猪13号染色体上。
