Fredholm M, Winterø A K, Christensen K, Kristensen B, Nielsen P B, Davies W, Archibald A
Department of Animal Production and Animal Health, Royal Veterinary and Agricultural University, Frederiksberg, Denmark.
Mamm Genome. 1993;4(4):187-92. doi: 10.1007/BF00417561.
Twenty-four PCR primer pairs were designed for the detection of porcine microsatellites. Polymorphism was investigated in 76 unrelated animals from four different breeds: Duroc, Landrace, Hampshire, and Yorkshire. Compared with human microsatellites, a general lower heterozygosity was detected; however, for each microsatellite a significant variation between breeds in number of alleles and heterozygosity was seen. Mean heterozygosity was found to be significantly higher (P < 0.01%) in the Yorkshire breed than in the other three breeds. Linkage analyses with the CEPH linkage packet were performed in a backcross family comprising 45 animals, of which 43 had informative meioses. Ten of the microsatellites could be assigned to six different linkage groups, demonstrating that linkage mapping with microsatellites can be carried out with great efficiency in a relatively small number of animals. Four of the linkage groups represent Chromosomes (Chrs) 4, 6, 7, and 8 respectively, while two linkage groups are unassigned.
设计了24对PCR引物用于检测猪的微卫星。对来自杜洛克、长白、汉普夏和约克夏四个不同品种的76头无亲缘关系的动物进行了多态性研究。与人类微卫星相比，检测到的杂合度普遍较低；然而，每个微卫星在等位基因数量和杂合度方面在品种间存在显著差异。发现约克夏品种的平均杂合度显著高于其他三个品种（P < 0.01%）。在一个由45只动物组成的回交家系中进行了与CEPH连锁包的连锁分析，其中43只动物有信息性减数分裂。其中10个微卫星可被分配到6个不同的连锁群，表明在相对较少数量的动物中，利用微卫星进行连锁图谱构建可以高效进行。其中4个连锁群分别代表第4、6、7和8号染色体，而另外两个连锁群未被分配。
