基于聚合酶链反应的猪胆囊收缩素（CCK）基因多态性及定位于13号染色体

Polymerase chain reaction-based polymorphisms in the porcine cholecystokinin (CCK) gene and assignment to chromosome 13.

作者信息

Clutter A C, Sasaki S, Pomp D

机构信息

Department of Animal Science, Oklahoma State University, Stillwater 74078-0435, USA.

出版信息

Anim Genet. 1996 Oct;27(5):369-70. doi: 10.1111/j.1365-2052.1996.tb00981.x.

DOI:10.1111/j.1365-2052.1996.tb00981.x

PMID:8930082

Abstract

Polymorphisms were identified in the porcine cholecystokinin (CCK) gene by digestion of products from polymerase chain reaction (PCR) with the restriction enzyme DpnII. Individuals from the European pig gene mapping project (PiGMaP) consortium reference families (eight full-sib families, 91 total progeny) were genotype to determine linkage relationships between the CCK gene and previously mapped loci. Linkage analysis revealed that the CCK gene is located on porcine chromosome 13.

摘要

通过用限制性内切酶DpnII消化聚合酶链反应（PCR）产物，在猪胆囊收缩素（CCK）基因中鉴定出多态性。来自欧洲猪基因图谱计划（PiGMaP）联盟参考家系（8个全同胞家系，共91个后代）的个体进行基因分型，以确定CCK基因与先前定位的基因座之间的连锁关系。连锁分析表明，CCK基因位于猪的13号染色体上。