Marcovistz R, Bertho A L, Matos D C
Departamento de Imunologia, Instituto Oswaldo Cruz, Rio de Janeiro, Brasil.
Braz J Med Biol Res. 1994 Jul;27(7):1599-603.
The apoptosis of thymocytes from rabies-infected mice was investigated in a kinetic study covering the entire course of the infection. For this study, BALB/c mice (6-7-week old females) were inoculated intracerebrally with 100 LD50 of Challenge Virus Strain, a fixed rabies virus strain, and three animals were sacrificed per time point to remove thymuses. When thymocytes were fixed, stained with propidium iodide and analyzed by flow cytometry, a distinct subpopulation of cells was observed below the G0/G1 region, denoted as the A0 region. Cells in this region presented reduced fluorescence, and nuclear DNA fragmentation. The accumulation of cells in the A0 region, after infection, progressively increased, reaching 12% for unfractionated thymocytes, 62% for thymocytes from the 60% Percoll interface and 32% for thymocytes recovered at the 100% Percoll interface. This finding, observed only in thymocytes from infected mice, demonstrates a clear modification of chromatin condensation in these cells, suggesting the occurrence of an apoptotic process during rabies infection.
在一项涵盖狂犬病感染全过程的动力学研究中，对感染狂犬病小鼠的胸腺细胞凋亡情况进行了调查。在本研究中，将6至7周龄的雌性BALB/c小鼠脑内接种100个半数致死量的攻击病毒株（一种固定的狂犬病病毒株），每个时间点处死3只动物以取出胸腺。当胸腺细胞固定后，用碘化丙啶染色并通过流式细胞术分析时，在G0/G1区域下方观察到一个独特的细胞亚群，记为A0区域。该区域的细胞呈现出荧光减弱和核DNA片段化。感染后，A0区域的细胞积累逐渐增加，未分离的胸腺细胞中达到12%，60% Percoll界面的胸腺细胞中达到62%，100% Percoll界面回收的胸腺细胞中达到32%。这一发现仅在感染小鼠的胸腺细胞中观察到，表明这些细胞中染色质凝聚发生了明显改变，提示狂犬病感染期间发生了凋亡过程。
