用于在醋酸纤维素膜上进行高压等电聚焦的热电冷却装置的研制。
Development of a thermoelectric cooling apparatus for high-voltage isoelectric focusing on a cellulose acetate membrane.
作者信息
Shiba K, Toda T, Iijima S, Inoue J, Yoshida T, Cho H, Kimura M
机构信息
School of Allied Health Sciences, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
出版信息
Biol Pharm Bull. 1994 Oct;17(10):1317-20. doi: 10.1248/bpb.17.1317.
To develop an isoelectric focusing apparatus using a cellulose acetate membrane (Separax EF), we have designed a thermoelectric cooling isoelectric apparatus. This apparatus has two characteristics. Firstly, the cooling system was switched to a thermoelectric cooling system from an ice-cooling system. Secondly, the chamber lid of the electrophoretic apparatus was also devised so that samples could be applied without opening the chamber lid. With this apparatus we could perform the isoelectric focusing without worrying about room temperature and humidity in the laboratory. Applying 2000 V for an extra 5 min with our module cooling system, we achieved a much higher degree of resolution with three sheets of cellulose acetate membrane (Separax EF) overlaid for simultaneous electrophoresis. Thus, three types of information could be obtained from only one electrophoretic procedure.
为了开发一种使用醋酸纤维素膜（Separax EF）的等电聚焦装置，我们设计了一种热电冷却等电装置。该装置有两个特点。首先，冷却系统从冰冷却系统切换为热电冷却系统。其次，电泳装置的腔盖也经过特别设计，这样在不打开腔盖的情况下就可以加样。使用该装置，我们在进行等电聚焦时无需担心实验室的室温及湿度。通过我们的模块冷却系统额外施加2000 V电压5分钟，我们在同时电泳时叠加三张醋酸纤维素膜（Separax EF）获得了更高的分辨率。因此，仅通过一次电泳过程就能获得三种类型的信息。
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