呼吸道合胞病毒糖蛋白G在脑膜炎奈瑟菌与HEp-2细胞结合中作用的证据。

Evidence for the role of glycoprotein G of respiratory syncytial virus in binding of Neisseria meningitidis to HEp-2 cells.

作者信息

Raza M W, Blackwell C C, Ogilvie M M, Saadi A T, Stewart J, Elton R A, Weir D M

机构信息

Department of Medical Microbiology, University of Edinburgh Medical School, UK.

出版信息

FEMS Immunol Med Microbiol. 1994 Nov;10(1):25-30. doi: 10.1111/j.1574-695X.1994.tb00007.x.

DOI:10.1111/j.1574-695X.1994.tb00007.x

PMID:7874075

Abstract

Viral glycoproteins G and F are expressed on the surface of cells infected with respiratory syncytial virus (RSV). We investigated the role of these proteins in the previously reported enhanced binding of Neisseria meningitidis to RSV-infected HEp-2 cells. Virus particles attached to bacteria were detected by immunofluorescence with flow cytometry. Binding of FITC-labelled bacteria to RSV-infected cells was significantly inhibited by monoclonal antibody against glycoprotein G. Unlabelled bacteria interfered with binding of the anti-G monoclonal antibody to these cells. These interactions were not found with a monoclonal antibody against glycoprotein F. We propose that glycoprotein G of RSV expressed on the surface of infected cells might act as an additional receptor for meningococci.

摘要

病毒糖蛋白G和F在感染呼吸道合胞病毒（RSV）的细胞表面表达。我们研究了这些蛋白在先前报道的脑膜炎奈瑟菌与RSV感染的HEp-2细胞增强结合中的作用。通过流式细胞术免疫荧光检测附着在细菌上的病毒颗粒。抗糖蛋白G单克隆抗体显著抑制了异硫氰酸荧光素（FITC）标记的细菌与RSV感染细胞的结合。未标记的细菌干扰了抗G单克隆抗体与这些细胞的结合。针对糖蛋白F的单克隆抗体未发现这些相互作用。我们提出，感染细胞表面表达的RSV糖蛋白G可能作为脑膜炎球菌的额外受体。