Shiomi S, Kuroki T, Ueda T, Takeda T, Nishiguchi S, Nakajima S, Kobayashi K, Yamagami S, Watanabe T, Minobe S
Third Department of Internal Medicine, Osaka City University Medical School, Japan.
J Gastroenterol. 1994 Dec;29(6):751-5. doi: 10.1007/BF02349282.
The Limulus amebocyte lysate (LAL) test has the disadvantage of being influenced by various inhibitors and activators. We have developed a method for the LAL reaction that involves the specific adsorption and isolation of endotoxin using a membrane filter unit and immobilized histidine; in this present study we used the method to measure endotoxin in the plasma of patients with acute or chronic liver disease. The adsorbed endotoxins are separated from LAL-inhibitors or -activators by the membrane filter unit, and their activity is directly assayed with the LAL reagent in a filter cup without any inhibition or activation. The study population consisted of 23 subjects, 3 with fulminant hepatitis and 20 with cirrhosis (9 with esophageal varices and 11 without). All 3 (100%) of the samples of plasma from patients with fulminant hepatitis were positive for endotoxin, as were the samples of 7 (78%) of the 9 patients with cirrhosis and esophageal varices, and 2 (18%) of the 11 patients with cirrhosis but without such varices. The results suggested that this method appears to be useful for assaying the concentration of endotoxin in patients with fulminant hepatitis or cirrhosis of the liver.
鲎试剂（LAL）检测法存在受多种抑制剂和激活剂影响的缺点。我们开发了一种鲎试剂反应方法，该方法利用膜过滤装置和固定化组氨酸对内毒素进行特异性吸附和分离；在本研究中，我们使用该方法测定急性或慢性肝病患者血浆中的内毒素。吸附的内毒素通过膜过滤装置与鲎试剂抑制剂或激活剂分离，其活性在滤杯中直接用鲎试剂进行检测，无任何抑制或激活作用。研究对象包括23名受试者，其中3例为暴发性肝炎患者，20例为肝硬化患者（9例有食管静脉曲张，11例无食管静脉曲张）。所有3例（100%）暴发性肝炎患者的血浆样本内毒素检测呈阳性，9例有食管静脉曲张的肝硬化患者中有7例（78%）样本呈阳性，11例无食管静脉曲张的肝硬化患者中有2例（18%）样本呈阳性。结果表明，该方法似乎可用于检测暴发性肝炎或肝硬化患者血浆中的内毒素浓度。
